and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special
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Yellow | ColourlessMedia: Pink | ColourlessMedia: Yellow | Pink-RedMedia: Pink | ColourlessMedia: Pink | E. coli grown in EMB agar: As we can see from the table above‚ the streak of E. coli on a plate with EMB agar showed a metallic green sheen where E. coli was present. EMB stain is selective for gram-negative bacteria. It is made using 6:1 Eosin and Methylene Blue. EMB agar is a differential media and inhibits the growth of gram-positive bacteria while also using a colour indicator to tell the difference
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TCBS agar is a selective as well as differential culture medium used for isolation of Vibrio spp. The growth of intestinal flora other than Vibrio spp. is prevented by the high pH (8.5-9.5) of TCBS. TCBS agar consist off yeast extract‚ bacteriological peptone‚ sodium thiosulphate‚ sodium citrate‚ ox bile‚ sucrose‚ sodium chloride‚ ferric citrate‚ Bromothymol blue/ thymol blue and agar. Sodium thiosulphate‚ sodium citrate and ox bile are the components
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of common household spices/herbs such as cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic‚ on the zone of inhibition in the Staphylococcus epidermis covered nutrient agar? Hypothesis: If 3 teaspoons of chopped cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic steep in half a cup of boiling water for 15 hours and then placed in nutrient agar covered with Staphylococcus epidermis (gram positive) bacteria for 24 hours (in an incubator)‚ garlic will have the strongest antimicrobial effect. This is because
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Bacteria will be grown in an agar broth containing .01%‚ .1%‚ .5% and 10% antibacterial soap. To fully understand the subject‚ information on everything necessary to conduct this experiment and comprehend the results was researched. Once the experiment is completed a conclusion will be able to be made as to whether or not bacteria can become resistant to antibacterial soaps The procedure for the experiment is as follows. Ecoli bacteria will be grown in agar broth with .01%‚ .1%‚ .5% and 10%
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Escherichia coli Microbiology Lab NAS 185-14L Introduction In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is
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Project 1: Antimicrobial Activity of Soil Isolates John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining
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Materials: 4 sterile cotton swab‚ sterile water in test tube‚ 4 agar plates and 2 blood agar plates‚ wax pencil and labels. Procedure: Appropriately label the cover of each plate as indicated in lab. 1. Determine 2 sampling sources‚ one from your body and one from the surrounding environment. 2. For the first agar plate‚ for sampling from air‚ remove the lids from the plate and allow it to sit uncovered for 15 minutes. 1. For second agar plate‚ open the “stick” end of the sterile cotton swabs to
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observable microbial colonies on the surface of the Agar solid‚ as to determine the presence of microbes in consumable products i.e. yoghurt and blue vein cheese. HYPOTHESIS: Microbial growth will be present in two of the three Agar plates (those containing the food product) due to the suspected presence of microbes‚ whilst the control Agar plate (containing no food products) will remain free of contamination and microbial growth. MATERIALS: - 3x Agar plates - 10g of berry yoghurt - 10g
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specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain‚ Catalase‚ Mannitol Salt Agar (MSA)‚ Blood Agar‚ Novobiocin‚ Coagulase‚ and DNAse (Alachi‚ 2007). Rebekah Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the
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