only for Penicillium citrinum whereas bark oleoresin has caused complete mycelial zone inhibition for Aspergillus flavus and A. ochraceus along with Aspergillus niger‚ Aspergillus terreus‚ P. citrinum and Penicillium viridicatum at 6 lL. Using agar well diffusion method‚ leaf volatile oil and oleoresin have shown better results in comparison with bark volatile oil‚ oleoresin and commercial bactericide‚ i.e.‚ ampicillin. Gas chromatographic–mass spectroscopy studies on leaf
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(Pearson’s 2011). Mannitol Salt Agar The Mannitol Salt Agar test was used to test only the Unknown Gram-positive bacteria. One colony from the Unknown 13A plate was used to inoculate the Mannitol Salt Agar Plate. After the plate was inoculated‚ it was placed in an incubator at 35ºC for 48 hours. If the agar surrounding the culture changed from red to yellow the test was positive‚ which indicated that the bacterium was capable of fermenting mannitol (Pearson’s 2011). If the agar surrounding the culture did
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Microbiology Exam 1 Name_______________________ 1/30/07 1. (1 pt) Who was the first person to observe bacteria using a microscope? a. Lister b. van Leeuvenhoek c. Pastuer d. Koch 2. (2 pts) Which two of the following contribute to the opportunistic and infectious nature of bacteria? a. flagella b. ability to persist in unfavorable environments c. selectively permeable membranes d. fast growth e. ability to sense chemical gradients f. peptidoglycan 3. (1 pt) Capsules‚ sheaths‚ and
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Purpose The principle of this experiment is to have a thorough perceptive of: • Culture washed and unwashed lettuce on agar plate. • Culture fresh and opened milk with the same expiration dates. • Explain the significance of food safety. • Illustrate foodborne sicknesses.
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ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and
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aeruginosa was gram-positive and therefore it would be susceptible. That was my hypothesis.We divided antiseptic agar plate into quadrants and divided antibiotics agar plate into six areas. We wrote the organisms’ name‚ date‚ and the temperature on the bottom of the agar plates. For antiseptic‚ our group used one color code for each quadrant. We streaked the swab in three directions on the agar plate and used sterilize forceps to remove filter paper discs from the container‚ and dipped it into solution
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reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase‚ catalase‚ lactose and sucrose fermentation‚ Kugler/iron agar‚ nitrate reduction‚ gelatin hydrolysis‚ starch hydrolysis‚ manitol salt‚ MR-VP‚ citrate‚ bile esculin‚ indole‚ urease‚ DNase‚ and coagulase. Material & Methods The tests performed on the unknown bacteria cultures were all used to determine
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Carbohydrate Polymers 95 (2013) 530–539 Contents lists available at SciVerse ScienceDirect Carbohydrate Polymers journal homepage: www.elsevier.com/locate/carbpol In vivo evaluation of chitosan–PVP–titanium dioxide nanocomposite as wound dressing material D. Archana a ‚ Brijesh K. Singh a ‚ Joydeep Dutta b ‚ P.K. Dutta a‚∗ a b Department of Chemistry‚ Motilal Nehru National Institute of Technology‚ Allahabad 211004‚ India Department of Humanities and Applied Sciences‚ Institute
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Case Study Report for Unknown #49 A new school for girls was opened in a larger village in Northern Pakistan‚ and the teachers were proud to have convinced many families in the surrounding villages to allow their daughters a basic education. The school was a success‚ and the 6-10 year olds girls quickly learned how to read and write and were even instructed in the sciences. However‚ 2 weeks before summer break‚ many of the girls and the teacher developed breathing problems that included severe coughing
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pigmentation‚ colony‚ margin characteristics‚ elevation properties‚ broth characteristics and agar stroke properties. 2. To examine bacteria growth characteristics on different culture media. Introduction: Bacterial species can sometimes be identified on the basis of how they appear on or in the different media. The pigmentation‚ size and shape of bacterial colonies as they grow on and in agar plates can provide identifying signs. Observing the growth characteristics of organisms in broth
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