semi-permeable outer membrane of E. coli would protect the bacteria from any antimicrobial properties of cilantro. A drop of cilantro juice and water in varying concentrations (1:10‚ 1:20‚ 1:40‚ 1:80) was added to a nutrient agar plate inoculated with S. epidermis and a nutrient agar plate inoculated with E. coli. The plates were incubated for 48 hours and then observed for a zone of clearing where the cilantro juice drop was placed. Cilantro was found to not display antimicrobial activity against either
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sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate. 5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate‚ making sure the lid of the culture container does not touch the surface‚ and that the lid of the agar plate is quickly replaced after this step has been carried out. 6. Sterilise the grabbing part and up the handle
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cells growing on soft agar burst from the viral infection and appears like a hole in the agar. Each plaque is created by the progeny of an individual phage and can thus be counted to determine the number of phage particles in a sample. The purpose of this lab is to employ a plaque assay method to determine the number of infected phage particles in the given sample. Method & Materials: * (6) BHI plates at 37˚C * (5) tubes of 9.0mL TSB broth * (6) tubes of soft agar in a 50˚C water bath
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CORE PRACTICAL ONE Describe how the effect of caffeine on heart rate in Daphnia can be investigated practically‚ and discuss whether there are ethical issues in the use of invertebrates. Daphnia‚ the water flea‚ is a small freshwater crustacean which lacks physiological methods of maintaining a constant body temperature. This means that if the environmental temperature changes‚ its body temperature does so too and its metabolic rate will be expected to rise or fall accordingly. So the temperature
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with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli EMB- pink mucoid colonies -------- K. pneumoniae e.coli (left) k.pneumoniae (right) *TSI ALK/Ag-----Serratia marcecens‚ Salmonella paratyphi A red slant‚ yellow butt with cracks or agar displacement or bubbles if with:
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Staphylococci‚ and Pseudomonas aeruginosa‚. The treatments include 100%‚ 75%‚ 50%‚ 25% and the controlled substances Chloramphenicol for Positive control and Pure Distilled water for Negative control. Antibacterial assay was performed using paper disc diffusion method. Zone of inhabitation were measured and compared among treatments means. Based on the result‚ the chemical was in extract concentration because they are steroidal that reduce the virulence or pathogenicity of cells in the body.
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Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA were used to inoculate Gram-positive tests‚ where as MacConkey Agar colonies were used to inoculate Gram-negative tests. Once the colonies were isolated and the appropriate Gram-negative and Gram-positive tests were conducted‚ the identification of both unknown organism were fairly easy. The results from the
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cold CaCl2 solution. Label one tube with your initials and a (+) and the other tube with your initials and a (-). 2. Transfer 2-4 large colonies using a sterile plastic loop to each microcentrifuge tube and completely resuspend. Do not transfer any agar. Put the tip of the loop into the CaCl2 solution and spin until there is not any cells on the loop. 3. Close each of the tubes and put them in ice. 4. Ask your teacher to
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Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
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purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials : 1) Blood agar plate . 2) Mannitol Salt agar (MSA) plate. 3) DNase agar plate . 4) Novobiocin disc . 5) Inoculating loop. 6) flame ( Bunsen burner) . 7) 1N hydrochloric acid (HCl) . 8) Two slides . 9) Plasma tube. 10) 3% Hydrogen Peroxide (H2O2) . 11) One unknown plate . 12) Crystal
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