microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized‚ a flame sterilized inoculating loop will be used to gather
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Carbohydrate Polymers 95 (2013) 530–539 Contents lists available at SciVerse ScienceDirect Carbohydrate Polymers journal homepage: www.elsevier.com/locate/carbpol In vivo evaluation of chitosan–PVP–titanium dioxide nanocomposite as wound dressing material D. Archana a ‚ Brijesh K. Singh a ‚ Joydeep Dutta b ‚ P.K. Dutta a‚∗ a b Department of Chemistry‚ Motilal Nehru National Institute of Technology‚ Allahabad 211004‚ India Department of Humanities and Applied Sciences‚ Institute
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semi-permeable outer membrane of E. coli would protect the bacteria from any antimicrobial properties of cilantro. A drop of cilantro juice and water in varying concentrations (1:10‚ 1:20‚ 1:40‚ 1:80) was added to a nutrient agar plate inoculated with S. epidermis and a nutrient agar plate inoculated with E. coli. The plates were incubated for 48 hours and then observed for a zone of clearing where the cilantro juice drop was placed. Cilantro was found to not display antimicrobial activity against either
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Reserved www.esciencelabs.com • 888.375.5487 Table of Contents Anatomy & Physiology Version 1 Preface: Introduc on to the Fetal Pig Lab 1: The Key to Reproducible Science Lab 2: Cell Structure and Func on Lab 3: Mitosis and Meiosis Lab 4: Diffusion and Osmosis Lab 5: Tissues and Skin Lab 6: The Skeletal System Lab 7: The Muscular System Lab 8: The Nervous System Appendix: Good Lab Techniques 3 Safety Informa on Lab Safety eScience Labs‚ Inc. designs every kit with safety as our
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sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate. 5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate‚ making sure the lid of the culture container does not touch the surface‚ and that the lid of the agar plate is quickly replaced after this step has been carried out. 6. Sterilise the grabbing part and up the handle
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with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli EMB- pink mucoid colonies -------- K. pneumoniae e.coli (left) k.pneumoniae (right) *TSI ALK/Ag-----Serratia marcecens‚ Salmonella paratyphi A red slant‚ yellow butt with cracks or agar displacement or bubbles if with:
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Day 1 Throughout the semester‚ I have learned multiple techniques for identifying bacteria. Learning how to gram stain‚ use specific media such as MacConkey agar‚ and test antibiotics to see which antibiotic would react best against a specific organism. All these techniques helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive
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cells growing on soft agar burst from the viral infection and appears like a hole in the agar. Each plaque is created by the progeny of an individual phage and can thus be counted to determine the number of phage particles in a sample. The purpose of this lab is to employ a plaque assay method to determine the number of infected phage particles in the given sample. Method & Materials: * (6) BHI plates at 37˚C * (5) tubes of 9.0mL TSB broth * (6) tubes of soft agar in a 50˚C water bath
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cold CaCl2 solution. Label one tube with your initials and a (+) and the other tube with your initials and a (-). 2. Transfer 2-4 large colonies using a sterile plastic loop to each microcentrifuge tube and completely resuspend. Do not transfer any agar. Put the tip of the loop into the CaCl2 solution and spin until there is not any cells on the loop. 3. Close each of the tubes and put them in ice. 4. Ask your teacher to
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Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
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