Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA were used to inoculate Gram-positive tests‚ where as MacConkey Agar colonies were used to inoculate Gram-negative tests. Once the colonies were isolated and the appropriate Gram-negative and Gram-positive tests were conducted‚ the identification of both unknown organism were fairly easy. The results from the
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TITLE PAGE PREPARATION AND CHARACTERIZATION OF SODIUM SALICYLATE OINTMENT INTENDED FOR TOPICAL AND SYSTEMIC DELIVERY BY MUOBIKE MAKUO 2005/136609 IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR THE AWARD OF DEGREE OF BACHELOR OF PHARMACY (B. PHARM) OF THE UNIVERSITY OF NIGERIA‚ NSUKKA. DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY FACULTY OF PHARMACEUTICAL SCIENCES UNIVERSITY OF NIGERIA‚ NSUKKA. PROJECT SUPERVISOR: DR. I. V. ONYISHI OCTOBER‚ 2012. APPROVAL PAGE This is to certify
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CORE PRACTICAL ONE Describe how the effect of caffeine on heart rate in Daphnia can be investigated practically‚ and discuss whether there are ethical issues in the use of invertebrates. Daphnia‚ the water flea‚ is a small freshwater crustacean which lacks physiological methods of maintaining a constant body temperature. This means that if the environmental temperature changes‚ its body temperature does so too and its metabolic rate will be expected to rise or fall accordingly. So the temperature
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purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials : 1) Blood agar plate . 2) Mannitol Salt agar (MSA) plate. 3) DNase agar plate . 4) Novobiocin disc . 5) Inoculating loop. 6) flame ( Bunsen burner) . 7) 1N hydrochloric acid (HCl) . 8) Two slides . 9) Plasma tube. 10) 3% Hydrogen Peroxide (H2O2) . 11) One unknown plate . 12) Crystal
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behaviors of different cellular slime molds were analyzed under different conditions. They found that in D. disciodeum thrived equally on nutrient soil and a non-nutrient agar dishes (Bonner & Lamont‚ 2005). Similarly to the experiment preformed by Bonner and Lamont‚ we evaluated growth patterns of D. disciodeum on non-nutrient agar dishes. Another study conducted by Fisher‚ found that certain genetic factors increase the movement and development of D. disciodeum amoebae within a temperature gradient
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nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of each petri dish and evenly spread bacterial culture around the agar plate. Cover and allow the culture to soak into agar for at east 15 minutes. Using sterile forceps‚ carefully place one filter disk from designated sample into the middle of each
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turn pink PEA prevents gram negatives from growing (2) PEA prevents the growth of Gram-negative organisms by disrupting the structure of lipids in the Gram-negative membrane. It also can hamper protein synthesis. Hemolysis is displayed on blood agar plates (2) On the plate the bacteria produces enzymes called hemolysins these enzymes lyse the red blood cells to certain degrees. Bacteria the fully lyse the blood go through beta-hemolysis. Bacteria that partially lyse the blood go through alpha-hemolysis
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2 agar plates divided into 4 equal sections were used for this experiment. Each section was labeled with a number from 1-8. 8 Sterile swabs were used‚ 1 for each surface swab. 8 surfaces in my home were then identified that could serve as a fomite and swabbed with a sterile swab that was dipped in distilled water to moisten it. Surface #1 was the garbage disposal in the kitchen sink. It was swabbed and the microbes transferred to the appropriately labeled section marked #1 of the agar plate
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allows the GFP gene to be expressed‚ but in both cases bacteria colonies would be present because of the gene of resistance to the antibiotic‚ ampicillin). We essentially made the required transformed solutions--and the controls--swiped them on the agar plate‚ and then observed to see whether or not bacteria colonies grew and whether or not they glowed. Our data fully supported our hypothesis. We can thus conclude that bacteria can take in foreign DNA through the process of transformation and that
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grew in many different ways. There were many different results in every bacteria that was examined‚ no bacteria looked alike towards one another. The bacteria in order to be produced it need to be put nutrient agar that would nourish it. The bacteria were inoculated into the nutrient agar so it can grow to be observed to view the results. Introduction Bacteria have been always around but us humans can’t see it with naked eye. Bacteria can be both harmful and good depending what type of bacteria
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