Your Full Name: UMUC Biology 102/103 Lab 1: Introduction to Science INSTRUCTIONS: On your own and without assistance‚ complete this Lab 1 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus). To conduct your laboratory exercises‚ use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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was place on a rack then the stain were apply. B. From agar cultures. 1. A drop of water was place in the center of the glass slide. 2. With sterilized inoculating loop to obtain a minute of bacterial culture from the agar cultures 3. It then mix with the drop of water and then proceeds as broth cultures. SIMPLE STAINING TECHNIQUES Material: 1. 24 hours broth cultures of a. E. coli b. Staph. aureus 2. 24 hours nutrient agar slants of a. Bacillus subtilis b. Pseudomonas aeruginosa
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various petri dishes containing agar‚ E Coli and the slime mold Dg‚ and the pH level is changed‚ then the petri dishes containing pH 4 (potassium acid phthalate) and pH 5 (sodium and potassium phosphate) will grow fruiting bodies more rapidly than pH 7 (potassium phosphate) and pH 9 (potassium phosphate and sodium borate)‚ since slime molds have a tendency to move away from ammonia‚ they probably do not prefer basic solutions. METHOD Materials: 4 petri dishes with agar and E Coli Dg pH 4 – potassium
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uses of bacteria. Throughout the investigation a series of experiments will be conducted to determine if metals such as nickel and aluminium have a resistance to E.coli K12 over a sequence of generations. To complete the investigation four Macconkey agar plates were inoculated with E.coli k12 and four paper discs of aluminium and nickel that were soaked in metal salts were placed into the plates to see the resistance that would occur over. Once resistance had occurred‚ the zone of inhibition was scraped
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Contributors to Bacteriology In an unassuming way‚. they moved agar from the kitchen to the lab‚ revolutionizing bacteriology WOLFGANG HESSE T RANSLATED BY D IETER H. M. GR~SCHEL Walther Hesse was a well-known community health physician in the Kingdom of Saxony‚ a student of Max von Pettenkofer‚ the father of hygiene‚ and of Robert Koch‚ the father of medical microbiology. His American wife‚ Fanny Angelina‚ introduced agar-agar to the new science of microbiology. The Hesse Family Walther
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In this lab you will test the idea that microbes are ubiquitous or present everywhere at all times. Materials One Triptic Soy Agar (TSA) plate per student One sterile swab Procedure 1. Decide on an area to test for the ubiquity of microbes. List your area on the white board in front of the classroom. 2. Obtain one TSA plate and label it on the bottom (side with the agar) with your name‚ class section and the surface you will sample. 3. Obtain one sterile swab. 4. To obtain a sample‚ roll the sterile
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in a color change of the medium from green to blue. Materials needed include simmons citrate agar slant tube‚ and a pure culture of bacteria. The starch hydrolysis test is used to determine the ability to hydrolyze starch. Bacteria use amylase‚ an extracellular enzyme to hydrolyze bonds linking the glucose subunits. Starch is detected by adding iodine to the medium. If the bacteria growing on the starch agar produce amylase‚ no color change will be seen around the bacterial growth because all of the
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Brain break- what type of medium is blood agar- COMPLEX Differential vs selective vs differential AND selective media Differential- ingredient added that can be changed by certain bacteria in a recognizable way Blood agar Hemolysis Alpha- green Beta- yellow surrounding strep throat Selective Hemolytic Ingredient added that inhibits growth f many unwanted organisms Antibiotic-containing medium Selective and differential MacConkey agar – gram neg that ferment lactose Bile
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utilizing procedures learnt during the semester. Procedures were followed as stated in the lab manual (1). Since the sample contained two unidentified bacteria‚ the first step was to isolate each bacterium using streak plate technique. Tryptic Soy Agar (TSA) plate‚ and differential media such as mannitol salt and Eosin methylene blue (EMB) were used for isolation streak technique. This step is imperative because the bacteria need to be separated and isolated before they can be identified. Moreover
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