Staphylococci‚ and Pseudomonas aeruginosa‚. The treatments include 100%‚ 75%‚ 50%‚ 25% and the controlled substances Chloramphenicol for Positive control and Pure Distilled water for Negative control. Antibacterial assay was performed using paper disc diffusion method. Zone of inhabitation were measured and compared among treatments means. Based on the result‚ the chemical was in extract concentration because they are steroidal that reduce the virulence or pathogenicity of cells in the body.
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bacterial walls. The solutions and fluids studied were saliva‚ mucus‚ tears‚ a stock solution of lysozomes‚ and distilled water. The solutions were placed in agar containing Micrococcus Luteus and we observed the amount of bacteria that was lyzed around them. The measurements were taken by observing where the agar cleared around the solutions‚ as the agar was cloudy where bacteria was present. I hypothesized that saliva would
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Materials • 22 prepared nutrient agar in petri dishes • Timer • Starburst candy (11) • 22 sterile swab • ½ slice of Bologna- Oscar Myer (11) • Sterile gloves G. Procedures 1) Prepare 4 petri dishes with nutrient agar. 2) Put on your sterile gloves 3) Pick up your first food (Starburst candy) and drop it on the ground. 4) Start the timer. 5) Pick up the Starburst candy from
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controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar upon feeding bacteria to it. 2. Why shoudn’t a student swab his or her mouth or cough onto an agar plate to initiate a culture? Even though most bacteria in the human body is harmless‚ if one was knowingly or unknowingly ill then harmful
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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Molecular Biology Lab Report Payton Jackson Introduction In this lab‚ I am going to use antibiotic-resistance plasmids to transform Escherichia coli. Materials For this lab you will need the following: LB Agar Petri dishes Beakers Test tubes CaCl2 solution Sensitive E. coli (-ampR) amp plasmids ampicillin -amp cells Water bath to heat shock cells A freezer to incubate cells Process Step 1: Wash hands and sanitize lab setting. This will prevent anything reacting with a
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Identification of Bacterial Pathogens basic skills in diagnostic bacteriology Dr.T.V.Rao MD Dr.T.V.Rao MD 1 Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface of Simmon’s citrate agar. Followed by overnight incubation at 37°C. Day after the slant
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