use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions: 1. Describe
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INTRODUCTION Plant pigments consist of four main chemicals. These are Chlorophylls‚ The carotenoids‚ Anthocyanin and Betacaine. The aims of this experiment is to separate the plant pigment into different colours using solvents and column chromatography. The hypothesis of this experiment is that this plant pigment from spinach will contain the chlorophyll pigment or may contain the others too. Chlorophyll: Chlorophyll a is the main component of the plant pigments. This is used for the most
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Diffusion Introduction Diffusion is the net movement of ions or molecules from a region of high concentration to a region of low along the concentration gradient until equilibrium has been reached. The steeper the gradient the faster the rate of diffusion. There are a few types of diffusion. Three (3) of those are: Facilitated Diffusion- refers to diffusion of substances across a cell membrane with the help of transport protein. Dialysis- refers to the diffusion of solutes across a semipermeable
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The Effect of Molecular Weight and Time on the Diffusion Rate of Potassium Permanganate‚ Potassium Dichromate‚ and Methylene Blue.1 Arantxa Alex Carpio Group 1 Sec. X – 4L March 24‚ 2015 ABSTRACT The effect of molecular weight and time on the rate of diffusion was determined using the agar-water gel test. A petri dish of agar-water gel with three wells was prepared and a prepared solution of each substance was dropped on each well; one with potassium permanganate (KMnO4)‚
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Biology 107 Section 1 11/4/13 Diffusion & Osmosis Introduction The purpose in this experiment was to estimate the osmolarity of potato tuber and celery cells by change of weight.Pieces of potato tuber and celery would be incubated in different sucrose solutions to find out the molarity at which weight of potato and celery tissues do not change. My hypothesis was that the Table 1: Group Data for Experiment:Estimating Osmolarity by Change in Weight Table
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Ronald Wilson Pd:4th 11/16/12 Introduction In this experiment diffusion and osmosis is the main idea. When using diffusion and osmosis you are trying to separate different solute concentrations on either side of the membrane. Only a solute’s relative concentration‚ or water potential‚ affects the rate of osmosis. The higher the concentration of solutes‚ the faster water will flow through the membrane to equalize the concentration. The way we describe the movement from higher to lower concentration
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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Experiment 5 Analysis of Plant Pigment Purpose: Chromatography is used for separation. In this case the separation of plant pigments. After placing the plant sample in the column the separation will be easy to see and the samples will be analyzed using VIS spectrophotometry. This is used to isolate and identify the different lipids causing the different pigmentations. Introduction: Lipids are chemically diverse substances found in cells. Lipids are water-insoluble‚ structural components
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Lab Activity No. __ Diffusion and Osmosis I. Introduction Diffusion is the transfer of molecules (or ions) from an area of higher concentration to a lower concentration‚ while osmosis is the transfer of water from a higher concentration to a lower concentration. II. Objectives 1. Demonstrate diffusion and osmosis across membrane. 2. Examine the relation of membrane permeability to diffusion and osmosis. III. Materials 2 thistle tubes‚ 2 big beakers‚ 4 tube clamps to fit iron stands
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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