Raw Data: • Table one; shows the effect of water temperature has on the rate at which the anthocyanin pigment leaves of a beetroot tap root cells • My groups results are highlighted in yellow and the class results are left blank Temperature ͦC (+/- 1 ͦC) Amount of green light that was absorbed by the solution (+/- 0.001) Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 20 0.099 0.188 0.202 0.141 0.152 0.143 0.157 30 0.274 0.173 0.205 0.170 0.262 0.180 0.233 40 0.285 0.127
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Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
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Plant Pigments and Photosynthesis Problem: We are going to separate and identify pigments and other molecules from cell extracts through a process called chromatography. We will also test the theory that light and chloroplasts are required for light reactions to occur. Background: Pigments are chemical compounds which reflect only certain wavelengths of visible light. This is what makes them seem colorful. Many things contain pigments including flowers‚ corals‚ and even skin. More important than
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Beach Channel High School Dr. David Morris‚ Principal The Living Environment Laboratory Study Guide The Living Regents Examination is at 9:00 a.m. on Friday January 26‚ 2007 Lab Activity 1 - Relationships and Biodiversity In this lab‚ students are introduced to classification and the importance of biodiversity. Organisms are according to similar characteristics. Some of these characteristics are physical (structural) and others are Biodiversity is the amount of the different organisms
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Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent material in the column separated the different pigments by allowing the least
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Hypothesis. If pigment from euonymus alatus (burning bush) leaves is extracted‚ then carotenes and xanthophylls will appear when a solvent‚ made of 9 parts petroleum ether and 1 part acetone‚ goes up Whatman #1 filter paper Purpose. The purpose of this experiment was to demonstrate the presence of carotenes‚ xanthophylls‚ and chlorophylls in leaves. Methods and Materials. A piece of Whatman #1 filter paper was cut to fit into an ignition tube. One end was cut to form a V‚ and the sides of
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BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
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This experiment focused on extracting and separating pigments of Chloroplast. For the procedure green leaves were grinded in a mortar with some chemicals and the fluid was filtrated to use for further analysis. Stripes of this solution were put on a filter paper and later‚ after dried placed into a beacon of solvent. After this the chloroplast pigments were separated by the solvent into groups of more or less soluble pigments. Aim How many pigment types are present in a green leaf? It is hoped to
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TABLE OF CONTENTS S no Topic Page number 1 Introduction 1 2 Objective 4 3 Scope and limitations 5 4 Theory 6 5 Experiment 11 6 Procedure 13 7 Observations 17 8 Result 18 9 Bibliography 19 INTRODUCTION Chlorophyll is a green photosynthetic pigment found in chloroplasts of organisms like cyanobacteria‚ algae and plants. Its name is derived from the Greek words chloros‚ meaning ‘green’ and phyllon meaning ‘leaf’. First isolated
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BRIEF INTRODUCTION: Beetroot cells contain a red pigment stored in the cell vacuole in the membrane to prevent leaking out from the cell. In this experiment we used several solvents to calculate which one made the cell release more pigment. RESULTS: A table showing the results of the intensity of the color and the absorbance of the liquids with the different solvents. Solvent Results from colorimeter 0.001 Acid (Hydrochloric) 0.358 Ethanol 0.132 Hot water 0.984 Cold water-distilled
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