LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Osmosis is the diffusion of water molecules from an area of high concentration of sucrose solution to an area of lower concentration of sucrose solution‚ through a selectively permeable membrane. The water molecules move down a concentration gradient in osmosis. Two diagrams of osmosis are shown in figures 1 and 2. Figure 1 shows the diffusion of water molecules through a selectively permeable membrane from an area of highly concentrated sucrose solution to an area of lower concentrated sucrose solution
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 or 23 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin was too large to diffuse into the right beaker. Activity 2: Simulating Dialysis 6. What happens to the urea concentration
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3.1 BIOLOGICAL MEMBRANES 3.1.1 Properties of Cell Membranes • Separates living cell from its nonliving surroundings. • 8 nm thick. • Selectively permeable - allows some substances to cross more easily than others. 4.1.2 Fluid Mosaic Model • Singer and Nicolson (1972) - plasma membrane is a mosaic of proteins dispersed within lipid bilayer‚ with only bilayer‚ the hydrophilic regions exposed to water. Hydrophilic region of protein Phospholipid bilayer Hydrophobic region of protein
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Membrane Transport Process Process Energy Source Description Examples Passive processes Simple diffusion Kinetic energy Kinetic energy Net movement of particles (ions. molecules. etc.) from an area of their higher concentration to an area of their lower concentration. that is. along their concentration gradient Movement of fats‚ oxygen‚ carbon dioxide through the lipid portion of the membrane‚ and ions through protein channels under certain conditions Osmosis Kinetic energy Simple diffusion
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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The plasma membrane of a cell is extremely important to the transportation of substances into and out of the cell. The outer membrane of a cell is made up of lipids and proteins. Most of the lipids in plasma membranes are phospholipids. The phospholipids have a hydrophilic head and two hydrophobic tails. When engulfed in water‚ they arrange themselves such that all the hydrophobic tails clump together‚ surrounded by the hydrophilic heads‚ protecting the tails from the exposure to the water. In cells
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able to diffuse into the dialysis bag. The Lugols (KI3) was able to diffuse through the membrane‚ because the molecules were small enough to pass through the semipermeable membrane and into the starch. The outside of the dialysis bag‚ with the Lugols and distilled
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process of diffusion of substances across a semipermeable membrane to gain a better understanding of how cells move materials across cell membranes. The experiment was designed to exhibit Brownian Motion; the random movement and tendency of particles of a substance to move from an area of greater concentration to lower concentration via diffusion until equilibrium has been attained. In addition to observing the passive process of diffusion‚ the experiment demonstrated how a semipermeable membrane will
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According to the data from both the lab group and the class average‚ there is evidence that osmosis did occur in the bags. The largest change in mass was in the 1.0M sucrose bag the mass went from 12g initially to 14.2g‚ this gained 2.2g‚ an 18.3% change in mass for the group data over the duration of the experiment. The 0.2M bag went from 10.2g to 10.9g a 6.9% change in mass; the 0.4M bag went from 12.1g to 12.2g .83% change in mass; the 0.8M bag went from 10.9g to 12.2g and an 11.9% change in
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