The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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Exercise 8: Chemical and Physical Processes of Digestion: Activity 1: Assessing Starch Digestion by Salivary Amylase Lab Report Pre-lab Quiz Results You scored 100% by answering 6 out of 6 questions correctly. 1. The substrate for amylase is You correctly answered: e. starch and carbohydrate. 2. Which of the following is true of enzymes? You correctly answered: c. Their activity can be affected by temperature and pH. 3. The reagent IKI tests for the presence of You correctly answered: a. starch.
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MARK333 Case Study – Cadbury Digestives Amanda Kelln 4969546 1. The ‘Thank You’ campaign worked so well because Cadbury did plenty of research on their target audience and then implemented an advert to deliver a particular message to this specific target audience. To be more specific‚ Cadbury determined from their research that their target audience was primarily women chocoholics who were looking to experience chocolate guilt free. Once the company determined this‚ they then implemented an advert
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DIGESTIVE SYSTEM Digestion is a process of breaking down complex food molecules into simple ones‚ suitable for absorption and their subsequent utilization in our body PURPOSE to convert complex food molecules into simpler ones‚ suitable for absorption and utilization production of energy‚ necessary for survival & vital chemical activity growth & development of the body repairing function reproductive function lactation FUNCTION to keep the
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What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However‚ different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body‚ there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it
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reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP) is a metal-containing enzyme that serves as a good model for the study of metal ion interactions in enzyme catalysis. Native E. coli ALP contains three
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The purpose of this essay is to research and evaluate the digestive system effectively and extensively. Then describe the differences and indifferences of three different organisms in relation to their digestive system. The three organisms that have been chosen are the human‚ for comparative purposes‚ the Goliath Bird Eating Spider and the Cobra Lily carnivorous plant. The Human Digestive System Digestion is the breaking down of substance in the body‚ into an easier form of which it can be absorbed
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it’s lowest to do so. However‚ in cold solutions the starch will take longer as it will in temperatures beyond 40 degrees. Once it reaches this point‚ the break down will either take a very long period of time or have no reaction at all as enzymes are denatured at a certain point. Materials: · 4 x test tubes · 5mL Diastase · 5mL Water · 10mL 2% Starch Suspension. · Pipette · 2 x Spotting tiles · Large Beaker filled with water of assigned temperature · Thermometer
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Nika Braiman 03/11/13 Digestive System Disorders Go to the following website: http://www.nlm.nih.gov/medlineplus/digestivesystem.html Review the structures of the digestive system using the interactive diagram. When you click on each digestive organ‚ a list of disorders related to that structure will be displayed. Complete the chart by matching the disorder with the structure involved with the disorder. Click on the disorder to get the description. Many disorders affect more than one structure
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Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
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