This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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Matthew Saldanha Bio DCP lab-Catalase experiment Aim: To investigate enzyme kinetics‚ using different concentration of the enzyme. Hypothesis: The assay system used in the lab consists of a filter paper disc coated with the enzyme and the dropped into a papercup of substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it
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-APPLICATION OF ENZYMES IN ORGANIC CHEMISTRY Why enzymes in organic synthesis? | Alternative to chemical methods: * High region- and stereoselectivity * Milder reaction conditions * Environmentally more friendly | Which enzyme(s)? | OxidoreductasesHydrolasesLyasesIsomerases | Which reaction system? | AqueousAqueous and water-miscible organic solventAqueous and water-immiscible organic solventPure organic solventOther solvents (supercritical fluids‚ ionic liquids) | Which ‘chemistry’
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contrast of a rabbit and tigers digestive systems Rabbits are herbivores that eat grass only. Rabbits can’t eat meat because it’s easier for them to digest grass. Tigers are carnivores that eat meat only. Tigers can’t eat grass because they cannot digest the food fast enough and their digestive system in the stomach is short. The digestive system of a tiger consists of an oesophagus‚ pancreas‚ mouth‚ stomach‚ small intestine‚ caecum‚ Large intestine. The digestive system of a rabbits consists of
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When rationally thinking about the gastrointestinal tract or digestion‚ nearly all of its functions are regulated by the autonomic nervous system‚ or involuntary‚ meaning that we do not consciously have to contemplate digestion for digestion to occur. With this being stated‚ it would make sense that almost the entire gastrointestinal tract is made up of smooth muscle. Smooth muscle is very distinctive from the other types of muscle in the body due to the fact that it is not controlled consciously
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Water‚ bile enzymes and mucus contribute to the change in consistency. The villi are essential for the absorption of digested nutrients. The cells of the villi transport food from the digestive tract into the bloodstream where they can be used by the body. The villi aid in absorption by increasing the surface area of the intestine and they contain specialized cells which transport different types of nutrients into the blood. The liver The livers main function within the digestive system is to
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results. For example‚ provide genetic and biochemical explanations to explain your results. Important: Students are required to hand-in their worksheets before leaving the class. Ensure that your name/ ID no. and sample number are recorded. The report should be written according to the sample problem provided in the handout. Question: Seeds of the F1 generation derived from a cross between two different yellow-seeded varieties of corn were collected as Sample F1. The F1 plants were selfed
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of an enzyme-controlled reaction. How do these factors affect the chemical structure and properties of the enzyme. Many things can affect the rate of enzyme activity. The temperature of the enzyme‚ the pH of the solution‚ the concentration of the enzyme‚ substrate and the product. Also‚ another affector is the number of competitive and non-competitive inhibitors. As I cannot explain them all‚ I have chosen to explain the effect of temperature and also the effect of inhibitors on enzyme activity
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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inhibits the brain’s ability to send signals to the spinal cord and nerve endings. The nervous system slows down greatly because it is absorbed right into the blood stream. Alcohol works the same as a sedative without the benefit of going through your digestive system. Because it enters directly into your blood stream‚ it is very dangerous. Your nerves control the speed in which your organs operate. They also manage your heart rate and breathing. And since you can’t control the way your nerve manage these
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