LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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The human body has to be controlled from advanced systems internally of which connect in order to function effectively. There are ten main systems within the body of which all perform various activities and all interrelate with at least one other system; the nervous system. The most complex of all body systems‚ the nervous system controls all functions within the body. The main function of the respiratory system is to inhale oxygen and exhale the waste product carbon dioxide. Oxygen is breathed
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The human digestive system‚ like those of other vertebrates‚ is built around an alimentary canal - a one way tube that passes through the body. The function of the digestive system is to convert foods into simple molecules that can be absorbed and used by the cells of the body. Food enters the mouth‚ where the work of the digestive system begins. Chewing‚ which takes place in the mouth‚ seems simple enough - teeth tear and crush the moistened food to a fine paste until it is ready to be swallowed
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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across fittings in a closed conduit to find out the loss coefficients of these fittings. Thereafter‚ we determine the individual flow rates of the 3 different lines in the system. With this knowledge‚ sufficient understanding about the properties and characteristics of the different devices that can be used in a piping system is necessary for the choice of the appropriate devices to be used in future experiments. In the second part of the experiment‚ we observe how the flow rate of a fluid changes
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are several different organ systems in the human body. Two of those systems are the digestive system and the urinary system. Both systems remove waste from the body but in an entirely different way. They also maintain homeostasis within our blood stream. Both systems are critical for survival. This paper will describe how each of those systems works on its own and also how they work together to maintain homeostasis for the body. How the Urinary and Digestive Systems Work Together to Maintain
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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example of crushing stone factory that how large stones are broken down into small particles in the factory and uses of these crushed stones in constructing roads‚ highways‚ bridge and buildings e.g. and prompted students to think about a system (digestive system) in the human body. Exploring: During exploration‚ students conduct various hands-on or problem solving activities and experiments designed to help them explore the topic and make connections to related concepts‚ often within groups or
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