starter plate needed contained the colony of E.coli needed for the experiment. A sterile innoculating loop was gently scraped over the colony and transferred into the tube with the calcium chloride‚ by gently twirling the tube in the solution. Using the capillary micropipette and plunger‚ 10 picometers of the plasmid PUc8 solution with the resistant gene was added to the tube labeled positive. Gently give a few taps to the tube labeled positive to mix the plasmid solution. Both of the tubes were placed
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Press‚ 1972. 5.Williams‚ Martin. Griffith‚ first artist of the movies. New York: Oxford University Press‚ 1980. 6.Nash‚ Bruce. 1997. The Numbers [online]. [cited 25 November]. Available from World Wide Web:(http://www.the-numbers.com/index.php) 7.Dirks‚ Time. 1996. Timeline of Influential Milestones and Important Turning Points in Film History [online]. [cited 23 November 2005]. Available from World Wide Web:(http://www.filmsite.org/milestonespre1900s.html)
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The FIFA World Cup Syafadzil : A very good morning to our English class teacher‚ Mr. Lee and to all my friends. Today‚ me and my friends‚ Amir and Firdaus are going to engage in a forum about the World Cup. Syafadzil : To explain it briefly‚ the World Cup is the world’s biggest sports event. It was held every 4 years since the first World Cup in 1930‚ except in 1942 and 1946 when it was not held because of World War 2. The tournament will be held in South Africa which involves 32 teams‚ each
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Introduction DNA (Deoxyribose Nucleic Acid) is a nucleic acid that has many names‚ each representing the phases that it undergoes (chromosomes‚ chromatin‚ genes/alleles); it resides in the nucleus (bound by 2 *phospholipid bilayers) of almost every cell in the body (red blood cells being an exception). DNA (your genotype) is double stranded and is responsible for replicating (from 46 to 92) during Interphase‚ so that mitosis can make new cells‚ repairing and allowing for growth in the body. It is
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the campsite‚ a fire will need to be started. After the fire is made‚ the marshmallows are now ready to be roasted. To roast a marshmallow‚ a person should remove just one out of the already opened bag. Next‚ he or she should place the marshmallow gently on the pointed end of the roasting stick. After that‚ the stick should be placed over the fire with the marshmallow on it. While
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after the three days? I think the egg will get bigger Procedure: (20points) a) Make two observations of the raw egg. b) Use the string and ruler to measure the circumference of the egg in cm. (Measure the egg around the smaller area.) c) Gently place the egg in the jar and cover it with vinegar. Put the lid on the jar. d) After approximately 24 hours from starting the experiment‚ make two observations of the raw egg. e) Carefully remove the egg from the jar
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Desires Joseph von Eichendorff is seen as one of the most famous poets of the German romanticism. Eichendorff composed many poems about his desires‚ the romantic world of joy‚ the simple intimacy‚ and the experience of nature as a way to God to create a mental antithesis to his violent affected military life. In this manner‚ his poem “Moonlight Night”‚ written in 1837‚ reflects his desires and his closeness to nature and to God. The speaker of the poem describes his feelings and impressions of
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smallest division of vernier scale is called the least count of vernier caliper. It may be different for different vernier calipers (and other measuring instruments). In order to measure length‚ put an object between the jaws and close the jaws gently. Fix the object with the fixing screw. (Fig.2b) In Fig. 2b‚ the leftmost thick mark on the sliding scale is between 9 mm and 10 mm‚ so the number of whole millimeters is 9. And number 3
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allow inoculating loop to cool before using inoculating loop to pick up‚ the liquid of the culture. * Agar plate to be open as little as possible‚ minimising possible access by bacteria from the air. * Using edge of inoculating loop move gently over surface of agar in a sweeping motion. * Place Antibiotic assay disc on top of the culture that has been spread on agar. * Seal agar plate with tape to guard against the possibility of anaerobic organisms growing due to lack of air.
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the brain was approached through the dorsal aspect of the skull. A T- shaped incision was given on the dorsal aspect of the head and the skin was reflected. The skull was nibbled avoiding damage to the brain. The brain was removed by releasing it gently from all its attachments from below and sides. Trigeminal ganglia were separated by cutting at the junction where the nerve enters into the brain stem. The brain was cleaned and washed with tap water followed by sterile water to remove excessive fixative
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