starter plate needed contained the colony of E.coli needed for the experiment. A sterile innoculating loop was gently scraped over the colony and transferred into the tube with the calcium chloride‚ by gently twirling the tube in the solution. Using the capillary micropipette and plunger‚ 10 picometers of the plasmid PUc8 solution with the resistant gene was added to the tube labeled positive. Gently give a few taps to the tube labeled positive to mix the plasmid solution. Both of the tubes were placed
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Desires Joseph von Eichendorff is seen as one of the most famous poets of the German romanticism. Eichendorff composed many poems about his desires‚ the romantic world of joy‚ the simple intimacy‚ and the experience of nature as a way to God to create a mental antithesis to his violent affected military life. In this manner‚ his poem “Moonlight Night”‚ written in 1837‚ reflects his desires and his closeness to nature and to God. The speaker of the poem describes his feelings and impressions of
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smallest division of vernier scale is called the least count of vernier caliper. It may be different for different vernier calipers (and other measuring instruments). In order to measure length‚ put an object between the jaws and close the jaws gently. Fix the object with the fixing screw. (Fig.2b) In Fig. 2b‚ the leftmost thick mark on the sliding scale is between 9 mm and 10 mm‚ so the number of whole millimeters is 9. And number 3
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allow inoculating loop to cool before using inoculating loop to pick up‚ the liquid of the culture. * Agar plate to be open as little as possible‚ minimising possible access by bacteria from the air. * Using edge of inoculating loop move gently over surface of agar in a sweeping motion. * Place Antibiotic assay disc on top of the culture that has been spread on agar. * Seal agar plate with tape to guard against the possibility of anaerobic organisms growing due to lack of air.
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the brain was approached through the dorsal aspect of the skull. A T- shaped incision was given on the dorsal aspect of the head and the skin was reflected. The skull was nibbled avoiding damage to the brain. The brain was removed by releasing it gently from all its attachments from below and sides. Trigeminal ganglia were separated by cutting at the junction where the nerve enters into the brain stem. The brain was cleaned and washed with tap water followed by sterile water to remove excessive fixative
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References: Dirks‚ T. (2010). The oscars 1990s [1994 Academy Awards Winners and History]. Retrieved April 8‚ 2011‚ from American Movie Classics Company LLC. website: http://www.filmsite.org/aa94.html Finerman‚ W.‚ Tisch‚ S.‚ & Newirth‚ C. (Producers)‚ & Zemeckis‚ R
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Introduction DNA (Deoxyribose Nucleic Acid) is a nucleic acid that has many names‚ each representing the phases that it undergoes (chromosomes‚ chromatin‚ genes/alleles); it resides in the nucleus (bound by 2 *phospholipid bilayers) of almost every cell in the body (red blood cells being an exception). DNA (your genotype) is double stranded and is responsible for replicating (from 46 to 92) during Interphase‚ so that mitosis can make new cells‚ repairing and allowing for growth in the body. It is
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hand supports the back of the kalimba. 2. What is the difference between a’smart’ and a’smart’? Dominant Hand Placement: Place your dominant hand over the top side of the kalimba‚ with your thumb resting on the higher-pitched tines and your fingers gently supporting the back of the instrument. Position your thumb and fingers comfortably‚ allowing
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tube‚ and two drops of 2-methylpropan-2-ol were placed in the the third test tube. 3) Under the fume hood‚ the teacher supervising the classroom added 10 drops of concentrated hydrochloric acid to each of the three test tubes. The mixtures were gently shaken. The test tube rack and test tubes were returned to the lab bench. The tubes were allowed to stand for one minute and evidence of cloudiness was observed. These observations were recorded. 4) The supervising teacher’s instructions were followed
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Speed ‚ V = ( λ = 2 ( (l2 - l1) Procedure : 1. Set the resonance tube apparatus vertical 2. Fill the tube with water 3. Select a tuning fork and strike it gently on a rubber cork. 4. Hold the vibrating prong horizontally over the mouth of the tube 5. While the tuning fork is vibrating‚ lower the reservoir. 6. Adjust the reservoir in such a position that a loud sound is heard.
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