sitting over a saucepan of gently simmering water and heat until the chocolate has melted. (Make sure the bottom of the bowl doesn’t touch the surface of the water.) Set aside to cool slightly. 3. Beat the butter until very soft then add the caster sugar and continue to beat until the mixture is pale and fluffy. Beat in the eggs‚ one at a time‚ then fold in the melted chocolate. 4. Sift in the flour‚ cocoa powder‚ baking powder and the bicarbonate of soda‚ and fold in gently to mix. Divide the mixture
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gram-negative cells will be pink. The methods for the Gram staining method first starts with a fixed smear of the bacterium‚ covered it with crystal violet for 30 seconds. Afterwards‚ it should be gently rinsed off with distilled water and be covered again with Gram’s iodine for 10 seconds. Then gently rinse off the Gram’s iodine with distilled water and decolorize it with ethanol. The ethanol should be rinsed off with distilled water again and then be covered with safranin for 30 seconds. The last
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With the barrel of her musket propped firmly on the limb of a tree‚ Charity took steady aim at the deer. The medium-sized buck was grazing at the edge of a thicket of wax myrtles‚ near where she saw the deer tracks the day before. John and Uriah were squatting in the bushes behind her. She had brought them along to help with the bleeding out of the deer‚ should she kill one‚ and to help carry it back to camp. They could then dress it out and help her prepare the meat for making jerky and so forth
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PNGase F is inhibited by SDS‚ therefore it is essential to have NP-40 in the reaction mixture under denaturing conditions. Failure to include NP-40 into the denaturing protocol will result in loss of enzymatic activity. 5. Add 1 µl PNGase F‚ mix gently. 6. Incubate reaction at 37°C for 1 hour. 7. Analyze by method of choice Note: The simplest method of assessing the extent of deglycosylation is by mobility shifts on SDS-PAGE gels. 2. Non-Denaturing Reaction Conditions: When deglycosylating a native
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Public Record Office Victoria PROS 10/10 Strategic Management Guideline Writing a Business Case Version Number: 1.0 Issue Date: 19/07/2010 Expiry Date: 19/07/2010 4 © State of Victoria 2010 Version 1.0 PROS 10/10: Guideline 4: Writing a Business Case Table of Contents 1. Introduction .....................................................................................................................4 1.1. Public Record Office Victoria Standards .............................
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Hardness Testing and Elastic/Plastic Deformation of Cantilever Beam February 9‚ 2012 Abstract This experiment examined the effect of a load placed on the end of a cantilever beam as well as the hardness of three different metals using the QV-700 Universal Hardness Tester. Introduction The overall purpose of this experiment is to become more familiar with experimental processes which will be used continuously throughout the semester. The first process was indentation hardness testing
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upholstery care guide upholstery care guide ACTIMA - RCS Nanterre B 400 009 981 all about leather YOU’VE JUST PURCHASED A ROCHE BOBOIS SOFA: You’ve been captivated by its sleek design and color; now you’re ready to discover the secrets beneath its elegant surface: how it was built‚ and how to preserve its fine quality. This manual will guide you through the care and maintenance of your ROCHE B OB OIS Sofa. THE ➀ ROCHE BOBOIS BEGINS AT THE TANNERY. BEAUTY‚ ALLURE‚ AND SUPPLENESS
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each tube One of the pair will gently blow on the solution in the test tube A while using the other partner times it. When the solution changes color the one timing it will “stop” and you will record your data in a table. Now‚ THE SAME STUDENT WHO EXHALED INTO TEST TUBE A IS THE ONE THAT will jog in place for 2 minutes. The timer will stay “stop” after 2 minutes and then RESET the timer Now the student that just finished exercising will blow into the test tube B gently with a straw while the other
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surfers gently sweep over the tumbling ocean waves. Seeing the tall palm trees slow movement back and forth in the wind is relaxing as they are making gentle‚ peaceful sounds. The terrace overlooks the tops green trees and vividly bright color flowers on one side‚ also a seemingly endless sandy beach and crystal clear water on the other side. During summer time‚ as anybody lies in the sand‚ he or she can see kites rise above the clouds and soar gracefully in the wind‚ which is blowing gently through
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Procedure: Preparation of t-Pentyl Chloride. In a 125-mL separatory funnel‚ place 10.0 mL of t-pentyl alcohol (2-methyl-2-butanol‚ MW _ 88.2‚ d _ 0.805 g/mL) and 25 mL of concentrated hydrochloric acid (d _ 1.18 g/mL). Do not stopper the funnel. Gently swirl the mixture in the separatory funnel for about 1 minute. After this period of swirling‚ stopper the separatory funnel and carefully invert it. Without shaking the separatory funnel‚ immediately open the stopcock to release the pressure. Close
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