blue onto the smear for 30 second‚ rinsed with water‚ blotted dry and observed the slide using oil immersion. Gram Stain For comparison purpose‚ I used two bacteria for this experiment. They are E. coli‚ a gram negative bacteria and Staphylococcus‚ a gram positive bacteria. I prepared a heat fixed smear of both bacteria. First‚ I used crystal violet as my primary stain‚ put few drops of it on the smear and let it sit for 30 seconds.
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Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
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My journey to the unknown started about three weeks before the end of my microbiology class at Brunswick Community College. This journey started out with a media plate cultured with an unknown species of bacteria. I was then instructed by my professor to use all of my previous knowledge to identify the unknown bacterial pathogen. This lab led me to master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with
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Bacteria are the most ancient life forms‚ most bacteria are so small that under a light microscope you can only see them as little dots. Some groups however grow to larger sizes and have spectacular shapes (1). Bacteria are present in most habitats on the planet‚ growing in soil‚ water‚ acidic hot springs‚ radioactive waste‚ and deep in the Earth’s crust‚ as well as in organic matter and the live bodies of plants and animals (2). Bacteria is mostly thought of as a pathogen‚ while it is true that
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The Abyss - Unknown Death. It is an odd thing‚ one moment you’re sitting in front of a desk pondering how to answer the question in front of you‚ the next extreme pain as if you’re entire body is being roasted alive. Then‚ well nothing. Just darkness‚ dull and boring. Those were the thoughts of the boy as he drifted in the abyss. His mortal coil no longer existence‚ only a dimly lit sphere in its place. “I’m not sure what’s worse‚ exams or this...” he muttered inwardly‚ originally he hadn’t been
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Lab: Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar
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is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature‚ pH level‚ and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria can also be used in many other
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repositories of bacteria/microbes ⁃ borne sterile ⁃ microbe on all surface area of the body ⁃ sterile areas: eyes‚ brain‚ spinal cord‚ bones‚ kidney‚ internal organs ⁃ mutualistic relationship: we provide site and nutrient and microbes provide vitamin‚ aid in food digestion ⁃ division of microbial world ⁃ living component: organism ⁃ prokaryotic ⁃ nucleoid area ⁃ one circular chromosome ⁃ non-membranous organelle ⁃ cell wall: peptidoglycan ⁃ binary fission ⁃ bacteria simple‚ dynamic
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The role of bacteria in the soil Bacteria in the soil play key role in recycling matter in to useful nutrients which can be used by growing plants. This process of recycling matter in the soil by living organisms is called biogeochemical cycle. Bacteria are improving plants growth in other ways too‚ for example bacteria‚ such as Rhizobium‚ are in symbiotic relationship with the root system of some terrestrial plants. The most important elements‚ which allow terrestrial plants grow well‚ are
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micro-organisms that are in the human digestive system. Article of choice‚ (Adnan‚ 2010) Benefits of Microorganisms to Humans‚ Retrieved February 8‚ 2013. This article was of interest because there are many bacteria that we encounter in our daily lives and it was informative to know that all bacteria is not bad. In fact‚ there needs to be a balance between the two and by educating yourself with the smallest amount of new information is very helpful. Humans rely on micro-organisms for a variety
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