The purpose of this experiment was to test how effective certain homogenates were as buffers. Buffers are devices that keep pH within maintainable boundaries so something can function. When something is too basic (has too much OH-) the buffer adds H+ and vice versa in order to create water to keep the pH at an acceptable range. Each group (I was with William Yung for this experiment) was tasked with testing one homogenate. The homogenate tested by our group was liquid spinach. Each team added HCl
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CHEMICAL REACTIONS: ACID-BASE BUFFERS Short Overview Acids and bases represent two of the most common classes of compounds. Many studies have been done on these compounds‚ and their reactions are very important. Perhaps the most important reaction is the one in which an acid and base are combined‚ resulting in the formation of water (in aqueous solution) and a salt; this reaction is called neutralization. A buffer solution is a solution that contains both an acid and a salt
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Purpose The purpose of this experiment is to determine the rate constants‚ k1‚ for the methyl acetate hydrolysis reaction at 25 °C and 35 °C‚ as well as the overall activation energy of the reaction. Methods Methyl acetate was placed in an HCl solution‚ in which it reacts with water to form acetic acid over time. At each time interval‚ an aliquot of the mixture was removed for titration against NaOH to determine the concentration of the acetic acid produced. From the amount of acetic acid produced
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1. Introduction 2 2. Graphs 2.1 HC2H3O2 titration curve 3 2.2 H3PO4 titration curve 4 2.3 H2A titration curve 5 3. Calculations 3.1 HC2H3O2 Calculations a. Exact molarity of the HC2H3O2 solution 6 b. Ka from the initial pH 6 c. Ka from the pH at halfway point 6 d. Ka from the pH at the end point 7 3.2 H3PO4 Calculations a. Exact molarity of the H3PO4 solution 7 b. Ka1 from
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E XPE RIME NT 4 . 5 Reactions of acids Aim To investigate and compare some reactions of a strong acid‚ hydrochloric acid‚ and a weak acid‚ ethanoic acid (common name‚ acetic acid) Equipment Dropper bottles containing: • 0.1 M hydrochloric acid‚ HCl • 0.1 M ethanoic acid (acetic acid)‚ CH3COOH • 0.1 M sodium hydroxide‚ NaOH • 1 M hydrochloric acid‚ HCl • 1 M ethanoic acid (acetic acid)‚ CH3COOH • universal indicator solution • limewater (calcium hydroxide‚ Ca(OH)2) Marble chips (calcium carbonate
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To identify an unknown sample‚ there were several steps that helped determine the molecular structure and the name of the sample. When Belle #9 (unknown) was given to me‚ it’s physical state and color was recorded on the data sheet. Since the sample was a pure liquid‚ there was no need for distillation. Next‚ a solubility test was given. When one drop of the unknown liquid was added to about 2 mL of water‚ the sample was tested to be soluble due to the dissolubility between the two compounds. By
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The full strength 1M HCl acid had a pH level of 3.12 (Table 3.1). Sample A2 to A4 remained in the pH 3 level‚ while sample A5 and A6 had a pH range of 4 (Table 3.1). The pH level for 1M HCl dilutions slightly increased between each sample (Table 3.1). The diluted solutions of 1 M NaOH showed a few unexpected results. The original solution of 1 M NaOH was at a pH level of 11.50 (Table 3.1). However‚ the pH of sample B2 increased to 12.81 (Table 3.1). The remaining samples had a decreasing pH pattern
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I. Introduction The purpose of this experiment is to determine the pH values of acids‚ bases‚ and buffers of distilled water and 10.0 buffer using measured concentrations of Sodium hydroxide (NaOH) and/or Hydrochloric acid (HCl). Acid is a compound typically having a bitter taste and capable of nullifying alkalis and releases hydrogen ion when added to a solution‚ or containing an atom that can accept a pair of electrons from a base (McKinley‚ Dean O’Loughlin‚ & Stouter Bidle‚ 2016). Bases are water-soluble
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In order to determine the acidity of certain substances‚ we used moist and dry pH paper (litmus tests)‚ and observed the results. First we used dry pH paper to test the acidity of household ammonia‚ and it turned yellow. According to the legend‚ this indicates a weak acid. This is‚ however‚ not accurate as the vapor of the household ammonia wasn’t able to react as strongly with the pH paper since it was dry. Then‚ we used moist DI water pH paper with the same substance‚ household ammonia‚ and it
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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