OBJECTIVES: The objective of this experiment was to extract plant leaf pigments and determining them by using the Rf values obtained from the paper chromatography technique. The hypothesis of the experiment was that all of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments used were a 18 x 150 mm test tube with stopper‚ graduated cylinders‚ Erlenmeyer flask‚ mortar and pestle‚ metric ruler‚ tall jar‚ acetone‚ tiny
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Experiment 1: Simple Distillation and Boiling Points- Separation of Liquids February 24‚ 2014 Analysis In this experiment‚ the distillation of three groups of two miscible liquids was performed. First‚ Ethanol and 2-Propanol were distilled. The boiling points of ethanol and 2-propanol had a difference of 5°C. The percent recovery for both ethanol and 2-propanol were both 0%. The percent recovery of the intermediate was 96%. The percent efficiency calculated of ethanol and 2-propanol was 0% efficient
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Search the web. Some interesting sites are listed below. Note that some of these sites go into much more depth than is reasonable for this course. http://en.wikipedia.org/wiki/Chromatography http://ull.chemistry.uakron.edu/analytical/Chromatography/ http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html this is for TLC – similar to paper http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/C/Chromatography_paper.html http://jchemed.chem.wisc.edu/JCESoft/Programs/CPL/Sample/modules/paprchrom/paprchromdesc
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Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart
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Title:The Chromatography Process of Expo Markers Abstract:This experiment is to uncover whether or not the Expo Markers are homogeneous or heterogeneous mixtures. The experiment will conduct filter paper being slightly submerged in a container of water and inspected for pigmentation changes. In the experiment the pigments will rise up the filter paper once exposed to the water (solvent). After the experiment‚ it concludes that the mixture is a homogeneous mixture due to the chemical
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plants. In this experiment‚ the essential oil eugenol was isolated from ground cloves using steam distillation techniques. Eugenol had a high boiling point‚ but it was isolated at a lower temperature by performing a co-distillation with water‚ a process known as steam distillation. This technique allowed the desired material to be distilled at a lower temperature than 100℃. The point of the steam distillation was to use an azeotrope to isolate the organic compound from a solid. The azeotrope was the
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Is Distillation? Answer: Distillation is a widely used method for separating mixtures based on differences in the conditions required to change the phase of components of the mixture. To separate a mixture of liquids‚ the liquid can be heated to force components‚ which have different boiling points‚ into the gas phase. The gas is then condensed back into liquid form and collected. Repeating the process on the collected liquid to improve the purity of the product is called double distillation. Although
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Distillation is a process in which compounds that are in the same solution become separated by their boiling points. For this to occur‚ a heterogeneous mixture has to be placed into a distiller‚ in this experiment a solution of 1:1 water and methanol was distilled. When distilling substances there are various methods of distillation that can be used. In this lab‚ simple and fractional distillation was used. The simple distiller is composed of a 100ml round bottom flask that is attached to: a
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Steam Distillation 6.6.12 Aim: To isolate and extract citrol from lemongrass oil using steam distillation. Theory: Impure chemicals are usually purified using distillation‚ allowing the changes in boiling points to separate the substances. Chemicals with high boiling points decompose before reaching the temperature necessary for separation‚ leading to the invention of steam distillation. The contamination is separated at lower temperatures and allows the separation of chemicals from their
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1 Section 2 – 12:30 to 2:20 Distillation and Fractional Distillation Distillation and Fraction Distillations Purpose: The purpose of distillation is to purify a liquid. Distillations are use to purify contaminates out of water to obtain clean pure water‚ as well as‚ to separate mixtures of liquids into their individual components; e.g. methanol and water. Objective: Distill methanol from water using a simple distillation apparatus and fractional distillation apparatus to determine which
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