each upstream and downstream fragments‚ 200 ng of pyrG marker fragment and adjust to 50 μL of double-distilled water. 35. Lysis Buffer: to prepare 50 mL of buffer dissolve 23.6 g of Guanidine thiocyanate (118.16 g/L) in 25 mL of double-distilled water. Once dissolved add: 2.5 mL of 1 M Tris-HCl pH 7.0 (121.14 g/L)‚ 2 mL of 0.5 M EDTA pH 8.0 (186.12 g/L) and 0.05 mL of Triton X-100. Add double-distilled water to 50mL (final concentrations: 4 M guanidine thiocyanate‚ 50 mM Tris-Hcl pH 7.0‚ 20 mM EDTA
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Control process Beetroot samples: The same size beetroots will be used throughout the experiment this is to ensure that the impact of the temperature on every sample will stay the same‚ i.e. if having a bigger beetroot sample less pigments will be released into the test tube at lower temperature‚ or having a smaller beetroot sample more pigment will be released into the test tube. This error will change the results of the experiment at large. Therefore it is important to insure the sample sizes
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(37’C)‚ a ruler‚ 2spatulas Materials: 1 gram of “Super clean” and “Magic power” washing powder‚ distilled water‚ iodine solution‚ a starch agar plate Procedure: i. Drill 3 identical wells on the starch agar plate by a cork borer ii. Weigh 1 gram of “Super clean” and “Magic power” washing powder separately using the electronic balance. iii. Dissolve the measured washing powders with 4mL distilled water in 2 test tubes respectively.. iv. Labeled the wells on the underside of the dish of agar
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beetroot and its cells. My earlier experiment suggest that as temperature increases the integrity of the beetroot cell membrane will be destroyed and a subsequent release of beetroot pigment will be released into its surrounding milieu(in this case distilled water). In the experiment I will examine if my hypothesis (above) is correct. In order to conduct this experiment I would choose a dependent variable as well as an independent one. The independent variable is the temperature at different degrees
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filter in the Buchner funnel. Wet the filter with distilled water so it sticks to the bottom of the funnel. e.) After the mixture from part (a) has separated‚ turn on the suction valve and then slowly pour the mixture through the filter in the Buchner funnel. Be sure to completely empty the beaker (use distilled water if necessary to wash the precipitate from the beaker). f.) Wash the precipitate in the filter with two 20-mL portions of distilled water. Allow the suction to continue for several
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What Effects Do Temperature And Standard Solution Have on a Beetroots Cell Membrane. Content: * Aim............................................................................................................. * Preliminary Work....................................................................................... * Hypothesis................................................................................................. * Risk Assessment.....................
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Petri dish 7 x 250 ml beakers (disposable cups may be substituted) sucrose solutions: 0.1‚ 0.2‚ 0.3‚ 0.4‚ 0.5‚ 0.6 molar marker razor blade/scalpel forceps cork borer balance (2dp) distilled water paper towels ruler (30 cm) Method 1. Get 100 ml of each of the solutions including distilled water. Place the solutions into the 250 ml beakers‚ and label them including your group names. 2. Use the cork borer to obtain seven complete potato cylinders at least 5.5 cm long. 3. Using the
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INTRODUCTION OF NIGERIAN AGIP OIL COMPANY Eni E & P Division commenced activities in Nigeria in 1962 through a wholly owed subsidiary NAOC Limited. Activities of Eni in Nigeria have grown over the years resulting in the establishment of other companies. These include the Agip energy and Natural Resources [Nigeria] Limited operating in the shallow water offshore and the Nigerian Agip Exploration Limited‚ which concentrates on the deep water frontier region. NAOC Limited operates in the land
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burned‚ cold water must be immediately applied to the injury. During disposal‚ keep gloves on to protect skin from the 10% bleach. Never ingest any liquids used during the experiment and always use gloves to protect the skin. Materials: Sterile distilled water (control) Steeped stick of cinnamon Steeped fresh cloves Steeped fresh mustard seed Steeped fresh ginger root Steeped fresh garlic Staphylococcus epidermis Nutrient agar plates (6) Micropipette Filter disks Incubator Strainer
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2 Plastic Film Containers (with lids) · Water · Stop Watch or Timer · Graduated Cylinder Procedure: Step 1. Put on apron and Safety goggles. Step 2. Get Alka-Seltzer tablets and plastic film container from teacher. Step 3. Measure x ml of distilled water with the graduated cylinder and pour it into the film container. Step 4. Drop x grams of Alka-Seltzer into the film container and close lids quickly. Step 5. Wait for reaction and record time and amount of water and Alka-Seltzer on the
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