Celecoxib (CLX) is a nonsteroidal anti-inflammatory drug (NSAID) used as a positive control for the antiangiogenesis assay Dimethyl sulfoxide (DMSO) an organosulfur compound with the formula (CH₃)₂SO which is capable of dissolving polar and nonpolar compounds. DPPH is also known as 2‚2-diphenyl-1-picrylhydrazyl. It is a stable free radical commonly used to evaluate the ability of compounds to
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Alternative cancer therapies are an emotive subject. In this Summative assessment the main events in a hypothetical case history are recounted from a naturopathic point of view and an effective management plan for the final part of the nutritional treatment is described. To support the given treatment strategy an attempt is made to subjectively consider a critique of nutritional therapies in general and the Gerson cancer therapy in particular‚ written by Saul Green PhD‚ biochemist and science editor
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C. partellus gut protease and protease inhibitor activity assays. C. partellus gut protease activity was estimated using chromogenic substrate BApNA [Manasi A et al.‚ 2005]. It was dissolved in the DMSO and a final concentration was made to 1mM in 1.0ml‚ Glycine-NaOH buffer (pH 9)‚ assays were carried at 38°C for 30min [Manasi A et al.‚ 2005]. The reaction was terminated by adding of acetic acid (30%) of 200µl [8‚ 9‚ Manasi A et al.‚ 2005 ]. Supernatant (0.5ml) was added to 1M NaOH (0.5ml) and the
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90%‚ respectively. Keywords: Cilnidipine‚ Chlorthalidone‚ HPLC. Cilnidipine (CIL) O3-(2-methoxyethyl) O5-[(E)-3-phenylprop-2-enyl] 2‚6-dimethyl-4-(3-nitrophenyl)-1‚4-dihydropyridine- 3‚5-dicarboxylate is off white crystalline powder soluble in DMSO‚ ethanol‚ methanol‚ water. Cilnidipine is L and N type calcium channel blocker used in treatment of hypertension due to its vasodilating effect(1). Cilnidipine is not official in any
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Exocytosis Lab Report Rough Draft Abstract: In Paramecium caudatum‚ trichocysts are 10^3 secretory vesicles that are released from the cytoplasm. We first observed how the presence of Alcian blue dye triggers normal secretion of trichocysts‚ and we then tested four different hypotheses in order to determine how the presence of Ca++ ions in the extracellular and intracellular environment affects the secretion of trichocysts. Our first our hypothesis tested whether extracellular calcium is
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Biological Activities of Paris polyphylla found in Himalayan Region of Nepal Prabin Koirala‚ Khagesh Chataut and Keshab Bhattarai Department of Biotechnology‚ WhiteHouse Institute of Science and Technology‚ Purbanchal University‚ Lalitpur‚ Nepal. e-mail: prabinkoirala122@gmail.com Abstract The study focused on phytochemical and biological analysis of Paris polyphylla (PP). Crude Chloroform/Methanol (1:1) extraction performed on the rhizomes of Paris polyphylla collected from himalayan region
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ABSTRACT Antibacterial activity of medicnal plants has turned out to be a global concern. Medicnal plants are very useful in treating various diseases. Antibiotics are an important class of pharmacological agents used for treating antibacterial infections. Although antibiotics were first isolated from fungi and bacteria but over the years more and more synthetic antibiotics has been synthesized‚ which are in great demand. This present study has been done to determine the antibacterial activity of
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Experiment 22 Laboratory to Biology III Diversity of Microorganisms / Wintersemester / page 1 Experiment 22 Isolation of plasmid-DNA from bacteria and PCR Advisor Konrad Egli: kegli@botinst.unizh.ch Reading Chapters in BBOM 10th: 10.8 BBOM : Madigan M.T.‚ J.M. Martinko and J. Parker: "Brock - Biology of Microorganisms"‚ 10th Edition (2003)‚ Prentice Hall. Objectives Background • Isolation of plasmid-DNA from different bacteria clones • Handling of bacteria clones
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independent variables by changing the amounts added and the order in which they were added to test what chemicals and what order they needed to be added to the reaction to produce a glow. Results: Trial Number | Luminol | Bleach | DMSO | 1M NaOH | 1M HCL | H2O2 | Results | A1 | Tad 1 | 1D 2 | 1D 6 S | 1D 5 | 1D 4 | 1D 3 | Turned brown | A2 | Tad 1 | 7D
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Cobalt(II) Oxygen adduct complex Inorganic 461 lab 2-29-2012 Abstract This experiment uses Co(salen) as a model compound illustrating the uptake of oxygen in hemoglobin. SalenH2 was prepared as the intermediate‚ and reacted with hydrated cobalt to prepare Co(salen). Using Co(salen) the oxygen up-take was tested. The synthesis of SalenH2 produced 0.65 g. The percent yield of salenH2 was 116% on the filter paper and 105% after transferring salenH2 to a vial. The melting point of salenH2‚ was
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