Article pubs.acs.org/jmc Thiazolopyridine Ureas as Novel Antitubercular Agents Acting through Inhibition of DNA Gyrase B Manoj G. Kale‚† Anandkumar Raichurkar‚†‚∇ Shahul Hameed P‚†‚∇ David Waterson‚†‚○ David McKinney‚∥ M. R. Manjunatha‚† Usha Kranthi‚† Krishna Koushik‚† Lalit kumar Jena‚† Vikas Shinde‚† Suresh Rudrapatna‚† Shubhada Barde‚† Vaishali Humnabadkar‚‡ Prashanti Madhavapeddi‚‡ Halesha Basavarajappa‚‡ Anirban Ghosh‚‡ VK Ramya‚‡ Supreeth Guptha‚‡ Sreevalli Sharma‚‡ Prakash Vachaspati
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2.3. Method Development 2.3.1. Preparation of Stock Solution 100 mg of the synthesized compound was made up to 100 ml into a 100ml standard flask with ethanol to give a solution of 1000 μg/ml. 2.3.2. Determination of λ max 1 ml of stock solution was pipetted out was made up to 10 ml into a 10ml standard flask with ethanol to obtain strength 100 μg/ml and scanned at 200-400nm using a UV spectrophotome-ter (Deepak V Bageshwar et al.‚ 2010). 2.3.3. Preparation of Standard Calibration Curve Aliquots
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recorded in DMSO on a Thermo UV1 spectrophotometer. IR spectra were recorded on a Perkin Elmer Spectrum SP-2 Fourier transform spectrophotometer using KBr pellets (4000–400 cm−1). 1H and 13C NMR spectra were recorded on a Bruker Avance II (400 MHz) FTNMR spectrometer using DMSO-d6 as solvent at 400 MHz and 100 MHz‚ respectively. TMS was used as internal reference for 1H NMR and 13C NMR. The 119SnNMRspectra with proton noise decoupling were recorded on a Bruker Avance II spectrometer using dry DMSO as the
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product was 3.329 g which comes out to be a 76.11% yield. The melting point of the product was 176.8-179.4°C. IR (nujol) 2500-3500 cm-1(-OH)‚ 2922.8 cm-1 and 2853.8 cm-1 (Sp3 C-H); H1NMR (d6-DMSO) 11.487ppm (2‚ 0)‚ 6.097ppm (M‚ 2H)‚ 3.160 ppm (D‚ 2H)‚ 2.995 ppm (S‚ 2H)‚ 1.280 ppm (S‚ 2H); 13C NMR (d6-DMSO) 173.979 ppm‚ 135.199 ppm‚ 48.714 ppm‚ 48.269 ppm‚ 46.384 ppm. After hydrolysis the Fisher Esterification reaction is ran on the diacid. In a 25 mL round bottom flask the endo-diacid is mixed
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General Chemistry Laboratory 151 Chemiluminescence Introduction: The objective of tis experiment was to carry out systematic series of experiments in order to determine which chemicals and which ratios of chemicals produce the brightest light. Also‚ this experiment will determine which chemical produces the longest light. Chemiluminescence is the emission of light (luminescence)‚ as the result of a chemical reaction. There may also be limited emission of heat. This occurs because the
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extraction was completed‚ the resulted essential oil was dehydrated using sodium sulfate (Merck). The essential oil was stored at temperature of 4ºC until using (12). In order to provide different concentrations of the extract‚ Dimethyl sulfoxide (DMSO) was used. Gas chromatography/mass spectrometry analysis of essential oil Isolation and measurement of the sample was done by coupled gas chromatography device (GC/MS) SHIMADZU 17 A with SHIMADZU mass spectrometry of QP5050A model and isolation of
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These investigated compounds were dissolved in DMSO at 10 and 20 mg ml-1‚separately [19‚21‚25‚27‚34]. Nutrient agar was prepared‚ then sterilized in an autoclave and poured in sterile Petri plates. After cooling of nutrient ager in Petri plates‚ the studied organisms were grown on agar. After that‚
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Vegetable is important commodities to human being because it provides vitamin and minerals. Due to the public concern regarding the importance of vegetables in their life‚ the public demand on the vegetables increase. For example‚ carrot‚ tomato and chilli are most demand among Malaysian adult. However‚ vegetables are susceptible to the microbial infection due to its pH that closer to the neutral pH. Generally‚ Alternaria‚ Fusarium‚ Penicillium‚ Aspergillus‚ Geotrichum and Botrytis are common post-harvest
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The SN2 reaction requires three qualities: a strong nucleophile‚ a good‚ unhindered leaving group‚ and a polar‚ aprotic solvent. For our reaction‚ we have all three bases covered. The nucleophile is an alkoxide‚ a deprotonated alcohol. Technically‚ because our alcohol is a phenol‚ the conjugate base is called a phenoxide. Phenol itself has a pKa of about 10‚ but our alcohol has more resonance opportunities‚ so the pKa is down around 8. This is sufficiently acidic for use of weak base like K2CO3 for
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inhibition [in mm] on agar plates) of sodium butyldithiocarbamate pentahydrate S. aureus (mm) E. faecalis (mm) E. coli (mm) BA 10.0 0.0 0.0 C5H20NNaO5S2 40.7 18.0 10.0 DMSO 0.0 0.0 0.0 Tetracycline 30 28 Vancomycin 22 BA: Butyl amine; C5H20NNaO5S2: Sodium butyl dithiocarbamate trihydrate; C=contamination Standards/Controls: DMSO‚ Tetracycline and Vancomycin and
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