Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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Abstract This experiment aimed to study the effect of various denaturants on albumin and casein protein extracts through viscosity measurements. 5 mL samples of native and denatured protein solutions were prepared‚ using -mercaptoethanol‚ urea and SDS as denaturants for albumin‚ and NaOH‚ NaCL‚ HCL‚ -mercaptoethanol‚ urea and SDS for casein. 5 mL blank solutions for each denaturant used were also prepared. The viscosity of the solutions were determined using Ostwald viscometer. ________
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This study investigated the effects of the inhibition Kinesio Tape technique on the triceps surae muscle after an isokinetic fatigue protocol. To the best of the researcher knowledge‚ there was only one study that used a fatigue protocol to study the effects of Kinesio Tape. This study was conducted by Yeung et al. (2014) and showed no significant difference in muscle strength when applying the facilitating technique of KT on the knee extensors before and after an isometric fatigue protocol. They
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DNA Sequencing As of last few weeks‚ the transformation lab is performed to convey and purify a given protein. However after further research scientists found out that Transformation is not only used to purify protein but also to find out contents that are stored in a given plasmid. The objective of the lab that is to be performed involves a procedure that determines the identity of an unknown gene replicated in a plasmid. To begin this procedure two to four colonies of bacteria is added to two
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. DNA is typically has two strands running in opposite direction and is usually referred to as a double helix. Each on the individual strands consists of a backbone that is formed by sugar molecules linked together in groups. Each individual sugar molecule is covalently linked to one of the following possible bases: Adenine‚ Guanine‚ Cytosine and Thymine. These bases
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TITLE: The Amount of Protein in Chicken Tissues over Cooked Various Periods of Time. ABSTRACT: In this lab‚ we are using a BioRad protein assay dye to determine the concentration of protein in our chicken. The dye binds to the amino acid residues‚ which allow us to find the concentration of protein (BioRad Protein Assay for Tissues). Our hypothesis was the longer chicken is cooked the less protein is available. To test our hypothesis‚ we made samples using our chicken and distilled water to determine
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DENATURATION OF PROTEINS Abstract The experiment aimed to use the concept of viscosity to study the effects of different denaturants on 1% albumin extract. An Ostwald viscometer was used to measure the flow time of 5 mL of the blank and native protein. These were then denatured by adding 1 mL of denaturant and had their flow time measured. The flow time from the blank to denatured protein is increasing. The specific viscosity and reduced viscosity
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Introduction: Protein texturization is a process of protein transform from a globular state to a fibrous physical structure that contains a sensation of eating meat. Texturized protein products have been defined as “fabricated palatable food ingredients processed from an edible protein source including among other soy grits‚ soy protein isolates and soy protein concentrate with or without suitable option ingredients added for nutritional or technological purposes.” (R.Y Yada‚ 2004) Protein products that
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and the discovery of DNA Ralf Dahm* Max Planck Institute for Developmental Biology‚ Department 3 – Genetics‚ Spemannstr. 35/III‚ D-72076 Tubingen‚ Germany ¨ Received for publication 5 October 2004‚ revised 17 November 2004‚ accepted 20 November 2004 Available online 21 December 2004 Abstract Over the past 60 years‚ DNA has risen from being an obscure molecule with presumed accessory or structural functions inside the nucleus to the icon of modern bioscience. The story of DNA often seems to begin
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HOW DOES SHAKESPEARE INFUSE ACT ONE OF ‘MEASURE FOR MEASURE’ WITH CONTEXTUAL REFERENCES? ‘Measure for Measure’ is set in Vienna‚ in 1603. It was written just after James the first‚ a protestant ruler came to the throne in England after the death of Queen Elizabeth‚ who was catholic. Religion is a big part of the play‚ especially in Act one‚ when the new proclamation is first introduced. Both Catholics and Puritans believe that sex before marriage is wrong‚ and during James the 1sts reign‚ the
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