TITLE: DNA Extraction from Banana DATE OF LABORATORY: 03/05/2013 LECTURER’S NAME: DR. LAM NYEE FAN DEMONSTRATORS’ NAME: MISS NOR EZANI AHMAD MISS LUSIA BAREK MOSES LABORATORY ASSISTANT NAME: MISS ROSILAH MOHD IDRUS STUDENT NAME AND MATRIC NUMBER: ELYAS ERIC HUIL(BS12110134) BONG SIN NENG(BS12110054) EDILAH NADRAH JOHANY( DIASSOFIA PAULA FRANKIE INTRODUCTION DNA is present in the cells of living oranisms. The nuclei acid DNA or deoxyribonucleic
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Charles Darwin: Pioneer of Evolution Theory Charles Darwin is the source of one of the biggest controversial subjects in the modern world. A pioneer of evolutionary biology‚ Darwin’s ideas and observations are all the more important in today’s further understanding of the sciences. Darwin’s work and understanding has propelled him to become one of the most influential scientists that ever lived. The Grandfather of Charles‚ Erasmus Darwin‚ was a physician and poet with a fascination for natural
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(ELECTROPHORESIS OF SERUM PROTEIN) DATE : 10 OCTOBER 2013 PART A SEPARATION OF SERUM PROTEINS USING THE ELECTROPHORESIS METHOD. OBJECTIVES: 1. To understand the analytical methods involved in analyzing serum proteins. 2. To study the serum protein electrophoresis pattern to aid the understanding of the relationship between the structure and the function of proteins. PRINCIPLE OF PRACTICAL: This technique is based on the movement of charged particles such as proteins when placed
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3.3 & 7.1 DNA Structure Worksheet 1. Draw and label the structure of a simplified single nucleotide‚ including sugar‚ phosphate and base. 2. Complete the table below to show the pairings of the bases in DNA: Purine | Pyrimidine | Adenine | Thymine | Guanine | Cytosine | 3. Where would one find the base uracil? RNA‚ it replaces thymine 4. In the space below‚ draw a single strand of three nucleotides‚ naming the bonds between them and showing the correct relative position
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Gene Cloning Methodology of DNA What is DNA? DNA was discovered by the Swiss biochemist‚ Johann Friedrich Miescher‚ in 1869‚ while he was working in Tubingen‚ Germany. He found that the DNA molecule is large; acidic in nature and rich in phosphorus‚ but only in the 1930s was the real and complex structure of DNA fully studied. DNA (deoxyribonucleic acid) is the genetic material in all prokaryotes and eukaryotes‚ i.e. it is the material responsible for the transfer of hereditary traits from
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Name Class Date RNA and Protein Synthesis Information and Heredity Q: How does information flow from DNA to RNA to direct the synthesis of proteins? WHAT I KNOW 13.1 What is RNA? 13.2 How do cells make proteins? 13.3 What happens when a cell’s DNA changes? 13.4 How do cells regulate gene expression? WHAT I LEARNED SAMPLE ANSWER: RNA is a nucleic acid that carries coded genetic information. SAMPLE ANSWER: SAMPLE ANSWER: The bases in DNA—A‚ T‚ G‚ and C—form a four-letter “alphabet” that
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The Some of the roles proteins play in the human body like Proteins serve as enzymes‚ help maintain the body’s fluid balance by attracting water‚ help maintain acid-base balance by acting as buffers‚ act against disease agents as antibodies‚ regulate body processes as hormones‚ transport nutrients and other molecules into and out of cells‚ help clot blood‚ help make scar tissue and bones‚ and serve as light-sensitive visual pigments. Also‚ Growth and repair this the most important and Individual
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suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your PCR? If the Taq DNA polymerase concentration is too low‚ not all DNA fragments will be fully replicated‚ which in turn reveals very faint bands. The optimal
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If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
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In the past protein powder has been the main product of choice of bodybuilders and other weightlifters who spend all of their time in the gym with the goal of becoming as strong and muscular as they can. But‚ in the past years‚ many other athletes and people have discovered the many benefits that protein powders have‚ causing the popularity of this product to increase rapidly. Many studies have proven that using protein powder while performing a resistance exercise plan will increase overall muscular
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