EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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DNA is the common name for Deoxyribonucleic acid. This is made up of nucleic acids containing deoxyribose (sugar)‚ consisting of complex molecules‚ present in the chromosomes of all plant and animal cells‚ and carrying in coded form instructions for passing on hereditary characteristics. The DNA molecule takes the shape of a double helix‚ a simple structure that resembles gently twisted ladders. The rails of the ladder are made of pairs of nitrogen-containing nucleotides‚ which are subdivisions
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plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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The DNA double helix model In 1953 James Watson and Francis Crick made a scientific breakthrough when they discovered the structure of DNA. From this structure they inferred that DNA contains information written in a genetic code‚ is passed from one generation to the next. This discovery was to be a key step in the genetics revolution DNA has four molecular building blocks called nucleotides; adenine (A)‚ thymine (T) guanine (G) and cytosine (C). Purine base always pairs (by hydrogen bonding) with
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company name] | DNA Research Paper | Coach Jones 6th period | | Austin Permann | 4/11/2011 | | Austin Permann 4/11/11 Period 6 Jones My DNA Research Paper DNA is something that is found in almost every organism. It is an abbreviation for deoxyribonucleic acid. It is the hereditary material in people and is located in the cell nucleus. DNA contains the genetic instructions used in the development and functioning of all known living organisms. DNA is pretty much
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Homework # 1 DNA 2. Name the four differences between RNA and DNA. 1) DNA is double stranded‚ RNA is single 2) DNA has Adenine‚ Guanine‚ Thiame‚ and Cytosine as nitro bases. With RNA‚ anywhere you would expect to see Thaime‚ you see Uracil instead. 3) DNA has no types‚ RNA has 3 types: mRNA‚ rRNA‚ and tRNA. 4) DNA and RNA have different sugars. 3. For the following DNS strand‚ provide the replicated strand : GATTACACATTAG CTAATGTGTAATC 4. For the following DNA sequence provide
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What role does DNA play in inheritance? - DNA is the genetic material of inheritance. Deoxyribonucleic acid (DNA) is the body’s instruction manual for making who you are. DNA is present in any living being. You receive one -half of your DNA from your money and one-half from you Father. People with light eyes tend to carry recessive alleles of the major gene and people with dark eyes tend to carry the dominant alleles. Genes are located on rodlike structures called chromosomes that are found in the
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Recombinant DNA Technology Recombinant DNA technology refers to the ability to isolate specific DNA sequences and alter or manipulate them to produce desired effects. More often‚ recombinant DNA technology is referred to as biotechnology. Recombinant DNA technology is fascinating in that it has developed into a multi-billion dollar industry‚ and completely revolutionized agriculture and pharmaceutical industries‚ all within the past 50 years. According to one account‚ biotechnology was born
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REPORT ON PROFILE STUDY ON DNA/HT’S EXCLUSIVE READERS. AT Bennett‚ Coleman & Co Ltd. The Times of India BY SAAD BHATI ROLL NO-P11014 PGDBM 3RD SEMISTER FOR POST GRADUATION DIPLOMA IN BUSINESS MANAGEMENT- MARKETING BATCH 2011-2013 CENTRE FOR MANAGEMENT – PGBDM BANDRA‚ MUMBAI – 400050 DECLARATION I Saad Bhati student of PGDBM - Marketing (Semester II) at Centre for Management hereby declare that this Project entitled “PROFILE STUDY ON DNA/HT’S EXCLUSIVE READERS”. has
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DNA STRUCTURE AND FUNCTION QUIZ Provide answers to each of the following. You may consult your text and fellow students. Each numbered item counts 2 points. 1. If one strand of a DNA double helix has the sequence GTCCAT‚ what is the sequence of the other strand? a. ACTTGC b. CUGGTU c. CAGGTA d. CAGGUA e. TGAACG 2. If a strand of DNA has the sequence AAGCTC‚ transcription will result in a(n) ______. a. single DNA strand with the sequence TTCGAG
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