BMS 424 Chapter 1 DNA‚ RNA‚ Historical Experiments & Structures Frederick Griffith Experiments with Streptococcus pneumoniae S. pneumoniae comes in two strains‚ smooth and rough strains S Smooth : Secrete a polysaccharide capsule; Protects bacterium from immune system of hosts; Produce smooth colonies on solid media. R Rough : Unable to form capsule; Produce colonies with a rough appearance. Griffith conducted experiments in 1928 using two strains of S. pneumoniae: type IIIS and type IIR :
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL Group partners: 1) HALIMATUN SAADIAH BT MOHD BUSTAMAM 2) NUR FARHANA BT AHMAD SOPIAN 3) FATIN NUR ASYIQIN BT ABD TALIB 4) UMMU AFIQAH BT HASSAN 5) NABIHAH BT MD NAWAWI Date of experiment: 8th October 2012 Date of submission: 15th October 2012 TITLE: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL DATE: 8th OCTOBER 2012 OBJECTIVE * To study measure the size of base pair of DNA RESULT Lane from extremely
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CHEM120 Week 7 iLab: DNA‚ mRNA‚ and Protein (30 points) Name: Kaylee Klefman Complete the two questions below. Each question has four parts. This assignment is two pages long. Question 1: For the following double-stranded DNA sequence‚ -CATTGACCGTAA- -GTAACTGGCATT- Answer the following questions: a) Assume that RNA polymerase will read the top strand of DNA as the “template” to synthesize mRNA. What will be the sequence of the mRNA synthesized? (3 points) The new mrna
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DNA is the primary genetic material that makes each individual different. DNA is the molecule that makes up chromosomes each pair of chromosomes contains DNA from one parent. DNA is found in the nuclei of eukaryotic cells and prokaryotic cells in living organisms. DNA is so important it is unable to leave the nucleus therefore the body makes RNA to carry information out into the cytoplasm. The three major components of DNA nucleotide monomers are phosphate‚ 5 carbon ring sugar (de-oxyribose) and
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DNA analyzing has been playing an increasingly role in the medical field. Genetic diseases’ rate is progressively growing but with the new technology‚ doctors are able now to reduce that high rate by using new invention. For example‚ with DNA analyzing doctors have the ability to reorganize the genetic disorder to make it better. Furthermore‚ now they can catalyze human body’s cells to produce a healing or therapeutic substance that could control the disease instead of fighting it. In another point
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Discovery of DNA Erwin Chargaff: Chargaff was an Austrian biochemist‚ he was best known for “Chargaff’s Rules” which lead to the discovery of DNA’s double helix structure. He immigrated to New York and secured a position as a research associate in the biochemistry department at Columbia University. Became a full-fledged professor in 1952. He used chromatographic techniques in his research. It was in the U.S.‚ 1950’s where he was able to make the crucial elements‚ in which lead to the formation
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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hospital where they take swabs from your vaginal area in hopes to collect enough Deoxyribonucleic Acid (DNA) to create a sample that can be added to the “Combined DNA Index System (CODIS)” (May & McIntyre‚ 2002‚ p. v). A few years have passed and you hear a knock on your front door. It is a police detective who has come to your home to tell you that an arrest has been made in your case based on a DNA match made from a mouth swab taken from a man who was arrested a few days earlier for robbery. The
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Can you expect to extract more DNA from a strawberry or kiwi fruit? Introduction: In the experiment we were inspecting the amount of genetic materials in fruits with different numbers of chromosomes. In the experiment‚ we mashed up a strawberry/kiwi fruit (See appendix 1 figure 2) added extraction butter and ethanol to extract the DNA. A chromosome is a long thread-like cluster of genes in the nucleus. DNA (deoxyribonucleic acid)‚ a self-replicating material which is present in nearly all living
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