To purify the PCR amplicon‚ both digested wt-goi and mut-goi from other components. A purified digested gene product is more advantageous for the DNA ligation‚ in which the gene products with sticky ends will be inserted to a plasmid vector. Also‚ to transform E.coli DH5α cells by introducing the plasmids DNA which contains the gene of interests into the E.coli strain(DH5α). The plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein
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There has been much debate in recent years among politicians‚ military officials‚ and civilians on whether or not the U.S. should be fully withdrawn from Afghanistan‚ regardless of the country’s economic and military capabilities. There has also been a proposed timetable‚ which President Obama says is in effect and currently being executed‚ to have up to ninety percent of our military personnel out of the country by no later than 2014. It is imperative that we extract our forces out of Afghanistan
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TRANSCRIPTION: Transcription is the process of which DNA matches corresponding RNA bases‚ Transcription is located in the Nucleus‚ and the only type of RNA that is involved in Transcription is mRNA‚ and the purpose is so that the code can get out of the Nucleus‚ mRNA is also made through Transcription‚ It also takes information that doesn’t directly make proteins but it helps makes codes for the production of proteins‚ DNA Transcription consist of 4 nucleotide bases‚ Adenine‚ Thymine‚ Cytosine‚ Guanine
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Extraction of Caffeine from Tealeaves Perez‚ Albert Solomon K.*; Quiambao‚ Marie Angela C;.Pascua‚ Hanna Harriette R.; Patricio‚ Maria Roxanne DC.; 2-Chemistry‚ Department of Chemistry‚ College of Science University of Santo Tomas España St.‚ 1015‚ Manila Abstract Caffeine is soluble in both water and organic solvents‚ solid-liquid extraction and liquid-liquid extraction were used in the experimentation process. Caffeine was extracted using hot water‚ however‚ due to its
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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Mohammed Awad Ms.Putnam English 2 1 December 2010 Louis Pasteur was born on December 27 1822‚ in a town called Dole Eastern France. He had three sisters and one brother that died when he was 1 years old. Louis Pasteur’s sisters names were Jeanne Antoine Pasteur‚ Josephine Pasteur and Jeanne Emile Pasteur. Jean Denis was his brother name and their mother name was Jean Pasteur. Their father owned a tanner and worked to make leather. His father was once in the army and won lots of
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keep you all. Amen. ABSTRACT The extraction of pectin from orange peels has been studied at different sample to solvent ratios‚ different PHs and with different extraction techniques using water. The amount of pectin extracted reduces as the PH increased. This research work is been carried out in order to determine the process condition that will result in high yield of pectin and residual oil extracted from orange peels using the methanol pre-treatment to extract orange
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Extracting DNA from Human Cheek Cells: Conclusion The hypothesis of my experiment‚ if the strawberry DNA product was strandlike yet clumply then human epithelial cell DNA will be strandlike and clumpy too‚ was supported. This is because through the observations noted when the lab was being conducted‚ it can be seen that the human epithelical cell DNA was small‚ stringy‚ clumpy and squishy‚ just like the strawberry DNA product. Other observations of the human epithelial cell DNA was it was not only
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Extraction of Caffeine from Thea sinensis Abstract Extraction of Caffeine from Thea sinensis main objective is to isolate‚ purify characterized caffeine from tea leaves. Sublimation technique was used to get the % yield which is 0.07%. The melting point of the standard caffeine with the sublimate is 229°C. Introduction The active ingredient that makes tea and coffee valuable to humans is caffeine. Caffeine is an alkaloid; a class of naturally occurring compounds containing nitrogen and
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Isolation‚ purification and characterization of alkaloid caffeine from tea leaves Date Submitted: September 3‚ 2014 Abstract Caffeine is one of the main constituents of tea leaves. In this experiment‚ 0.0039 g of pure caffeine was isolated from tea leaves. This was done through solid-liquid extraction followed by multiple liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together
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