The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
Premium Bacteria Escherichia coli DNA
this lab was to troubleshoot causes related to PCR components and develop an experiment that would test if the Taq amount is suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your PCR? If the Taq DNA polymerase
Premium DNA Bacteria Gene
Effect of Incorporation of Carrot Powder in Wheat Flour for the Preparation of Stick Noodles Kripa Shakya1 and Anup Halwai2 1.College of Applied Food and Dairy Technology‚Kathamndu‚ Nepal 2. Department of Food Technology and Quality Control‚ Kathamndu‚ Nepal krips16@hotmail.com Abstract Noodles were prepared by incorporating carrot powder in the percentage of 5‚ 10‚ 15 and 20 in wheat flour. The main objective of the work was to prepare the nutritious noodle enriched with Vitamin A. Sensory
Premium Flour Vitamin A Pasta
Isolation of Acetyleugenol (Clove Oil) from Cloves Purpose In this experiment‚ you will obtain the essential oil from cloves by steam distillation. You will then separate a component (acetyleugenol) from the oil by liquid-liquid extraction. Finally‚ you will isolate the acetyleugenol by using a rotovap for reduced pressure distillation‚ which will remove the organic solvent (dichloromethane). Background Whole cloves contain between 14% and 20% by weight of essential oils‚ but
Premium Distillation
Ethanol Production from waste Banana peelings and its use as a source for fuel. Rajiv Gandhi University of Knowledge Technologies Abstract: This study entitled “Ethanol Production from Waste Banana Peelings” aims to produce an alternative way in producing energy. Production of green energy from waste material has played an important role in recent days due to the
Premium Waste Ethanol Waste management
SEGi University Extraction of Benzoic Acid by Balvinder Singh (SCM 015 677) Lab Partners: 1) Surrean Rao 2) Wong Kai Jun 3) Kanaga Luckshimi 4) Sasiskala Regal ECE2332 Chemical Engineering Practices Submitted: 1st October 2013 Supervisor: Mr Ayman William 1.0 Abstract /summary The purpose of this experiment to carry out in the lab is because to determined the capability to water droplet to carry out the liquid-liquid extraction. The studies is to determine
Premium Concentration Liquid Chemistry
there is nothing stronger than a bond between a father and his son. The trying and heartwarming relationship between the father and his son is shown in the story “Powder” by Tobias Wolff. There are many differences and similarities between the father and his son‚ maybe a few more differences than similarities but that doesn’t stop them from spending time with each other. To begin with‚ throughout the story the reader makes it clear the differences between the two characters. First off‚ the father
Premium Family Mother Tobias Wolff
EXTRACTION OF CAFFEINE FROM TEA LEAVES ABSTRACT Caffeine extraction from the commercial tea leaves (Lipton Yellow Label Tea) that was done is multiple extraction. 4 tea bags were used in the experiment; tea leaves were weighed having 8.5333 grams. The leaves were boiled in a beaker with 150ml of water; the aqueous tea extract was transferred in a seperatory funnel mixed with DCM (20ml)‚ done thrice. The DCM layer was washed with 20ml 6M NaOH in the seperatory funnel; the organic layer
Free Caffeine Coffee Tea
“Powder” "Powder‚" by Tobias Wolff‚ is about a man recalling his memory of a specific ski trip taken with his father‚ at a time when his parent’s were on the verge of divorce. He recounts the day with vivid detail‚ telling us the reasons why his parents divorce was imminent‚ but also how much he loves his father despite his faults. The boy conveys his controlling behavior to the point of numbering his clothes to ensure rotation‚ and asking for school assignments well in advance so that he can
Premium Tobias Wolff Mother Anxiety
Extraction of caffeine from Lipton tea leaves Maria Gianna Beatrice L. Cancio*‚ Joe Mari Isabella B. Caringal‚ Rowena A. Chiang‚ Patricia Deanne del Valle Department of Biological Sciences‚ College of Science‚ University of Santo Tomas‚ España‚ Manila‚ Philippines Abstract Caffeine was extracted from Lipton tea leaves by multiple extraction techniques namely solid to liquid extraction and liquid to liquid extraction; the purification by sublimation and melting point determination. Three
Premium Caffeine Solubility Tea