DNA helicase -separates strands of nuclei acid‚ breaks H bond between nitrogenous bases.‚ works at the replication fork -DNA PRIMASE- lays RNA primer ‚ acts as new strand‚ can only add nucleotides to a free3’ end ‚ lays nucleotide with a 5’ orientation -DNA POLYMERASE 3- adds nucleotides using base pair rules lcreating 2 new daughter strands‚ only adds to a free 3’end and lays down nucleotide with 5’ orientation. Pol3 continuously synthesizes new daughter cell(leading strand) same direction as
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Extraction and Sublimation of Caffeine from Tea Leaves By: Ashley Barnes CH 236 – P8 Experiment 4 & 4.5 Due: 10/15/2014 Table 1. Table of Reagents Discussion The extraction yield refers to the percent of caffeine in the tea leaves at start of the extraction experiment. Tea leaves had 0.55 grams of mass per eight ounce serving. The weight of the extracted crude caffeine was 0.264 grams. Therefore‚ the extraction yield was 48%. This means that we pulled out almost half of the caffeine
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Jimmy Wales and Michael Snow. | [Hide] [Help us with translations!] | DNA vaccine What is antisense technology? Antisense refers to opposing the normal order (“sense”) of the code in DNA. The DNA (deoxyribonucleic acid) in genes directs cells to assemble the proteins which comprise living creatures. The order of bases in DNA corresponds to the ordering of amino acids to form the proteins. To produce protein‚ the DNA of the genes in cells is first transcribed into a very similar molecule called
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Extraction (Ch # 07) Faraz Gohar Mr. Wayne Xie Chem 201 10/02/2012 Objectives : 1)To purify samples of organic compounds that are solids at room temperature. 2)To dissociate the impure sample in the minimum amount of an appropriate hot solvent Chemical Index : Naphthalene; mp 82 *C 1‚4-Dichlorobenzene; mp 56 *C 4-Chloroaniline; mp 68 - 71 *C; pKb 4.15 Ethyl 4-Aminobenzoate; mp 90 *C; pKb 4.92 Procedure :
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The upper most strand is the (coding strand) DNA base sequence (triplet) of the gene codes for synthesis of a particular polypeptide chain. The second strand is the mRNA base sequence (template strand used for copying) codon of the transcribed mRNA. The process for going from the upper stand to the second strand is called Transcription and involves an enzyme called polymerase. The polymerase attaches to the promoter region (start codon) and reads the nucleotide base sequence until it gets to a termination
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the quantification of albumin and casein were performed and analyzed after extraction of the said proteins from their respective sources. Isolation of proteins was initiated by the breakage of the cell wall / membranes in three different ways. Homogenization of invertase‚ albumin and casein were achieved via grinding process‚ addition of 1M acetic acid and acidification by 0.1M hydrochloric acid correspondingly. Extraction of invertase and casein involved precipitation through the utilization of 95%
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2.3.3 Feature Extraction Feature extraction deals with the extraction of the distinctive features out of face images so that those face images can be differentiated among each other. There are several algorithms available for extracting features out of a face image. The most common is the use of mathematical formulas that generate a mathematical representation of a face image that is termed as a template‚ these templates are “the refined‚ processed and stored representation of the distinguishing
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Experiment 3 Liquid-Liquid Extraction Discussion The success of extracting each compound out of the solution can be seen in the IR graphs for each substance. For ethyl 4-aminobenzoate‚ the N-H‚ C=O and C-O are distinguishable in their proper wavenumber positions. The amino N-H stretch was found between 3224 cm-1 and 3423 cm-1. The C=O was found at 1681 cm-1 and the C-O was at 1280 cm-1. The IR for benzoic acid also displays its significant bonds‚ O-H and C=O. The O-H was between 2566 and
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DNA molecules are very long. They wrap around proteins and wind tightly‚ forming structures called chromosomes. A human somatic (non-sex) cell has 23 pairs of chromosomes. Twenty-two pairs are autosomes‚ which do not differ between the sexes. The autosomes are numbered from 1 to 22‚ with 1 the largest. The other two chromosomes‚ the X and the Y‚ are sex chromosomes. The Y chromosome bears genes that determine maleness. In humans‚ a female has two X chromosomes and a male has one X and one Y. Charts
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DNA Extraction from Wheat Germ and Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled
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