DNA EXTRACTION FROM KIWI ISGR SCIENCE Aalah Yousif‚ Ella Sobek April 27‚ 2012 Background All living organisms (plants‚ animals‚ and bacteria) are made up of cells. Any cell consists of many parts but the parts that play the key roles are the nucleus‚ cell membrane‚ and cytoplasm. The nucleus is like the “brain” of the cell (that also contains DNA). The cell membrane is like a wall that guards the cell. And the cytoplasm is a jelly-like substance which contains other smaller organelles like
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Can you expect to extract more DNA from a strawberry or kiwi fruit? Introduction: In the experiment we were inspecting the amount of genetic materials in fruits with different numbers of chromosomes. In the experiment‚ we mashed up a strawberry/kiwi fruit (See appendix 1 figure 2) added extraction butter and ethanol to extract the DNA. A chromosome is a long thread-like cluster of genes in the nucleus. DNA (deoxyribonucleic acid)‚ a self-replicating material which is present in nearly all living
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Report August 28‚ 2013 DNA Extraction on Onion (Allium cepa) and Human Cheek Cell Arriza‚ Rolland Merch Buscato‚ Carl G. Butil‚ Conrad G. Leonida‚ Renee Theresa ABSTRACT This activity observes the DNA present in onion (Allium cepa) and human cheek cells by extracting it with the addition of lysis buffer and chilled ethanol. The lysis buffer is prepared from squeezed onion mixed with salt and Pantene Pro-V Shampoo. The buffer degrades the protein enveloping the DNA found in onion and cheek
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Kiwi (Actinidia deliciosa) Family: Actinidiaceae Synonyms: Actinidia chinensis Other names: Chinese gooseberry ------------------------------------------------- Top of Form Bottom of Form Custom Search Kiwi is among the very few recent introductions which have surpassed in popularity due to its tremendous commercial potential in the sub Himalayan region of India. A native to central China‚ it is being grown commercially in New Zealand‚ Italy‚ USA‚ Japan‚ Australia‚ France‚ Chile and Spain. In
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learned from studying an organisms DNA. The first step to doing this is extracting DNA from cells. In this experiment‚ you will isolate DNA from the cells of fruit. Materials (1) 10 mL Graduated Cylinder(2) 100 mL Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh‚ Soft Fruit (e.g.‚ Grapes‚ Strawberries‚ Banana‚ etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou Must
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Extraction of DNA Science Fair Project 2013-2014 Produced by Nadia Walker Due Date: Monday January 20th 2014 Presented to Mr. Freimann Date Submitted: Monday January 20th 2014 Table of Contents Introduction page 1 Hypothesis and materials
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Bioinformatics Leigh Ann Santana DNA Extraction Lab DNA extraction is an important process because the DNA first needs to be purified away from proteins and other cellular contaminants. Cell are needed‚ because that is where the DNA is located. Inside almost every cell in our bodies is a nucleus‚ and inside each nucleus is about two meters of DNA. The following steps are needed to purify DNA from a cheek swab. Collect cheek cells‚ Burst cells open to release DNA‚ separate DNA from proteins and debris
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DNA EXTRACTION In extracting chromatin from the cells of wheat germ there are seven steps to follow. The optimal cell to use would be the polyploidal eukaryotic. Eukaryotes have nucleus membrane-bound organelles‚ while prokaryotic does not. The polyploidal eukaryotic cell has DNA that is held in the nucleus while the prokaryote has DNA that floats freely around the cell. The DNA of eukaryotes is more complex and extensive than the other. Prokaryote is a bacterial cell that
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DNA Extraction from Fruit 1. What was the purpose of adding liquid soap and salt in step #1 and how does NaCl contribute to maximum DNA extraction. The purpose of using soap was to destroy the membranes inside a kiwi cell. Soap helped with that because it dissolves the membranes easily. Salt or NaCl was used to remove proteins and carbohydrates. NaCl caused the proteins and carbohydrates to precipitate. 2. Why was it necessary to “mush” the kiwi by hand? If the step was omitted‚ what
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Objective : To extract the DNA from onion samples. Introduction : Nuclues has DNA molecule in it. It is packaged into thread like structure known as chromosomes. Each and every chromosome is made up of DNA tightly coiled many times around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides
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