Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed
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DNA Lab Report SungYong Jang What is DNA? What do the letters stand for? What is it composed of? Where is it found? What is it shaped like? Answer in full sentences. It is the genetic material that can be passed on from parent to offspring DNA --> Deoxyribonucleic acid It composed of Deoxyribose (5 carbon sugar)‚ a phosphate and a Nitrogonous base (the 4 N-bases are Guanine‚ Cytosine‚ Adenine and Thymine) It is a double helix (looks like
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placed in an electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel matrix. In visualization‚ the gel is dyed so the results become present. This is used in modern crime labs‚ figuring out DNA‚ which plays a key role in many criminal trials. The researcher completed this experiment to figure out who committed the murder using gel electrophoresis. The researcher followed five steps in this experiment; First was placing the gel in
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wberry DNA Lab ReportMeredith Anderson Mrs. Berman Biology October 17‚ 2012 Extracting DNA from Strawberries Problem/Question: Successfully extract the DNA from strawberries. Theory/Hypothesis: N/A Variables: Independent – strawberries Dependant – the DNA Controlled – 450 ml of water‚ 50 ml of soap‚ 1 tsp of salt‚ 5 ml isopropyl alcohol Apparatus and Materials: N/A Procedure and Control of Variables: N/A Data Collected: See attached paper. Data Processing and Presentation:
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Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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3.2.4 Genomic DNA extraction of the root tips After the treatment of the onion bulbs with lake water sample at different period of time‚ the DNA from the root tips will be ready to be extracted and observed. First of all‚ the genomic of the Allium cepa will be easily done by the already available Uneasy® Plant Mini kit (Qiagen‚ Germany) in the laboratory. All of the procedures can be performed by referring to the manual provided by the kit. A very small amount of root tips that weigh 100 mg will
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We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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IN EMBALMING. A DEAD-END ROAD TO FORMALDEHYDE-FREE CHEMICALS.. [ONLINE] Available at: http://www.themodernembalmer.com/tannin.html. [Accessed 07 October 14]. 4. Science Lab. 2013. Material Safety Data Sheet Caffeine MSDS. [ONLINE] Available at: http://www.sciencelab.com/msds.php?msdsId=9927475. [Accessed 07 October 14]. 5.Science Lab. 2013. Material Safety Data Sheet Dichloromethane MSDS. [ONLINE] Available at: http://wcam.engr.wisc.edu/Public/Safety/MSDS/Dichloromethane.pdf. [Accessed 07 October 14]
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Anza Thomas 10/24/2013 CHE 261 (KC) Dr . McAtee Lab : Extraction of Caffiene Purpose: the purpose of this experiment is to extract caffeine from tea using solvent extraction techinques. Reference: Williamson‚ K.; Minard‚ R.; Masters‚ K. Macroscale and Microscale Organic Experiments‚ 2007‚ p. 137 to 171. Procedure: In a 50 mL beaker place 15 mL of water‚ 2.0059 g of sodium carbonate‚ and a wooden boiling stick. Bring the water to a boil on the ceramic heating plate. Remove the beaker
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