Abstract The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided‚ and then recrystallize the caffeine. The solvents used in the experiment were an aqueous sodium carbonate and dichloromethane (DCM). Anhydrous calcium chloride pellets were used to dry the solution and emulsion layer and the DCM was then decanted. After washing the anhydrous calcium chloride pellets with more DCM‚ the solvent was evaporated‚ leaving
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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Organic Lab Report Extraction Separation of a Carboxylic Acid‚ a Phenol‚ and a Neutral Substance Purpose The objective of the experiment is to identify the three substances in the mixture and to determine the percent recovery of each from the mixture. The unknown will consist of either benzoic acid or 2-chlorobenzoic acid‚ 4-t-butyl phenol. Determine the weight of crystals and also the percent yield Introduction There are various techniques of extractions‚ solid/liquid
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the Extraction lab is to separate and purify benzoic acid‚ 2-naphthol‚ and naphthalene. These organic solids are purified by partitioning the solid in 2 immiscible solvents‚ diethyl ether and sodium bicarbonate or sodium hydroxide. II. Significance a. The significance of the Extraction lab is to purify Acetanilide benzoic acid‚ 2-naphthol‚ and naphthalene by determining the partition coefficient. This value is determined by dividing the solubility of the given solute in the extraction solvent
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SEGi University Extraction of Benzoic Acid by Balvinder Singh (SCM 015 677) Lab Partners: 1) Surrean Rao 2) Wong Kai Jun 3) Kanaga Luckshimi 4) Sasiskala Regal ECE2332 Chemical Engineering Practices Submitted: 1st October 2013 Supervisor: Mr Ayman William 1.0 Abstract /summary The purpose of this experiment to carry out in the lab is because to determined the capability to water droplet to carry out the liquid-liquid extraction. The studies is to determine
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Lab 5 Acid/Base Extractions Introduction The purpose of this experiment is to separate either the organic base (amine) or organic acid (carboxylic acid) from a mixture that contains inorganic impurities (salt) by performing a liquid-liquid extraction and then taking a melting point. Key Experimental Details and Observations Our starting material‚ Compound B‚ was a fine white powder and weighed 0.535g. The final product was a shiny white sheet that resembled acrylic paint and weighed 0.109g
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DNA extraction in Watermelon and Bananas Abstract During this experiment the researcher investigated the differing amounts of DNA present in bananas and watermelon. So that the researcher could do this‚ and accurately record the amounts‚ the researcher liquefied the fruits in a blender‚ added water and salt to them‚ and the added laundry detergent before using contact solution as an enzyme. Rubbing alcohol was then poured into the mixture and the DNA was extracted and
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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hydrocarbons‚ PCBs‚ pesticides‚ herbicides‚ and other organics. Page 1 of 3: Analytical Result A. Header Information 1. After "Project No:"‚ enter the client’s project number (from cover page 1). This number is required on every page of the report. 2. After "METHOD"‚ enter the analytical method used. (e.g.‚ EPA 8260‚ or EPA method 8021). 3. After "REPORTING UNIT"‚ enter the appropriate reporting unit. The units ug/L for water samples and ug/Kg for soil samples are recommended for volatile
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