SOP: Solid phase extraction of cylindrospermopsin in filtered water samples Document identifier: SOP_TOXIC_UDU_05F Prepared by: James S. Metcalf and Geoffrey A. Codd‚ UDU Date: 7 July 2005 1 Introduction In order to determine the concentration of cylindrospermopsin in the extracellular fraction of filtered environmental waters‚ solid phase extraction (SPE) is necessary to concentrate the toxin to concentrations capable of being detected by HPLC. 2 Experimental 2.1 Materials
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In the book Ella Minnow Pea by Mark Dunn he writes a fictional novel to entertain his reader while trying to bring out a bigger message. His message is evidently shown throughout the whole book. The message Dunn wants to address in his book appear to be that we as individuals are lacking language‚ communication and critical thinking and the consequences that come with it. Mark Dunn uses epistolary to write this book which makes the reading more personal‚ for the reader is reading letters between
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called extraction. Extraction is a systematic process of separating mixtures of compounds‚ taking advantage of the affinity differences of compounds to separate them (Padias 128-37). This technique recognizes the principle that “like dissolves in like‚” that is‚ polar solutes dissolve in polar solvents and non-polar solutes dissolve in non-polar solvents. Through extension of this principle‚ one can use extraction to separate compounds of a mixture. There are three different methods of extraction; solid-liquid
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Introduction Extraction is a purification technique used in organic chemistry to separate compounds from a mixture of two or more compounds. There are three different extraction techniques: liquid-liquid extraction‚ solid-liquid extraction and chemically active extraction. All three types of extraction follow the same principle. Organic molecules dissolve in organic solvents and polar molecules dissolve in aqueous solvents. This phenomenon is observed because of the intermolecular forces between
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Experiment title: Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page
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I am choosing the book‚ The DNA of Relationships‚ because as a Christ follower‚ we are meant to come together‚ as a community‚ to worship and love of Lord. However‚ if aspects of healthy relationships are not present‚ the outcome of the environment will be detrimental to the relationships built with one another‚ and even possibly God. We each‚ as individuals‚ are unique in or capabilities and attributes; therefore‚ we are meant to be in community with one another in order to combine and form a new
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its mixture using acid-alkaline extraction. 2. To determine the percentage recovery and melting point of benzoic acid and p-diclorobenzene. Apparatus:Separatory funnel(250mL)‚Buchner funnel‚beaker. Materials:Benzoic acid‚p-dichlorobenzene‚ether‚10% NaOH‚conc.HCl‚distilled water‚ anhydrous CaCl2. Introduction: Organic compounds in an aqueous mixture can be separated by shaking the aqueous solution with a solvent which is immiscible with water. Extraction involves dissolving either a
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DNA Lab Report SungYong Jang What is DNA? What do the letters stand for? What is it composed of? Where is it found? What is it shaped like? Answer in full sentences. It is the genetic material that can be passed on from parent to offspring DNA --> Deoxyribonucleic acid It composed of Deoxyribose (5 carbon sugar)‚ a phosphate and a Nitrogonous base (the 4 N-bases are Guanine‚ Cytosine‚ Adenine and Thymine) It is a double helix (looks like
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Abundance and Extraction of Metals 1. a Mineral - A mineral is a naturally occurring inorganic solid‚ with a definite chemical composition‚ and an ordered atomic arrangement. b Ore - Deposit in the Earth of valuable metals contained in minerals. c Economic Natural Resource Deposit - This is a natural deposit of something that is economically viable to mine‚ refine and sell. Hence the deposit must be big enough to last for a large period of time‚ so that it will make enough money for the
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Definition of Terms The following terms were defined for the readers to have a better understanding of the reserch paper. Active constituent. It refers to the component of the plant responsible for the exhibitory activity of the drug.1 Agar. It is gelatinous colloidal extractive of a red alga (as of the genera Gelidium‚ Gracilaria‚ and Eucheuma) used especially in culture media or as a gelling and stabilizing agent in foods.2 Agar-well diffusion method.3 It is a method of bioassay wherein
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