Discussion Although only the extraction of strawberry DNA was performed in this lab‚ this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well‚ and compare it to the steps taken in the strawberry protocol. As described in the procedure using strawberries‚ the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective
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Deoxyribonucleic Acid (DNA) DNA stands for deoxyribonucleic acid‚ it is located in the nuclei of cells which make up the body. DNA is quite often referred to as one of the building blocks of the body.. It is made up of four bases known as: • Adenine • Guanine • Cytosine • Thymine James Watson‚ Francis Crick‚ Maurice Wilkins‚ Rosalind Franklin Crick and Watson‚ together with Maurice Wilkins‚ won the 1962 Nobel Prize in physiology or medicine for their 1953 determination of the structure of
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Since its discovery in the 1980’s‚ the application of DNA has been utilized in many ways. When Deoxyribonucleic Acid‚ or DNA is examined‚ a precise decoding of genetic information is revealed. With the exception of identical twins‚ every person’s genetic code (commonly referred to as a Genetic Fingerprint) is inherited and unique. From Maury advising men everyday on TV “You ARE the Father!” or “You are NOT the Father!” to learning the descendents of former slaves are related to President Thomas Jefferson
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the structure of DNA. ➢ DNA is a nucleic acid‚ which consist of long chains (polymers) of chemical units (monomers) called nucleotide. A molecule of DNA contains two polynucleotides‚ each a chain of nucleotides. Each nucleotide consists of a nitrogenous base‚ a sugar‚ and a phosphate group. Each DNA strand serves as a mold‚ or template‚ to guide reproduction of the other strand. There are four different types of nucleotides found in DNA‚ differing only in the nitrogenous base. DNA is contained in
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DNA Technology: Biotechnology 1) Selective breeding a. 2) Genetic engineering – humans tinker with organisms genes a. Cloning –take haploid cel and replace with a diploid nucleus (comes from organism whos traits you want to duplicate. b. Recombinant DNA – DNA from 2 or more sources. Done by Euk cells during Meiosis. Always from same molecule. 3) Biologists first started doing recombinant DNA from a prok cell and combined it with another prok cell because it was easier. 4) DNA from a
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TAMARINDUS Linnaeus pp. 434-436‚ Manila * Srivastava‚ P. & Malviya‚ R. (2011) Indian Journal of Natural Products and Resources Vol. 2 (1) Sources of pectin‚ extraction and its applications in pharmaceutical industry. pp. 10-18 * May‚ C. D. Handbook of Hydrocolloids pp. 169-188 * Huang‚ J. M. G. Improved method for the extraction of Pectin‚ PO Box 4200 Highstown‚ New Jersey 08520 Acknowledgement I would like to thank God‚ for His guidance throughout this investigatory project. To my
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History of DNA WebQuest 1. Friedrich (Fritz) Miescher http://www.dnai.org/timeline/index.html Find Miescher on the timeline and click on the bucket with the Red Cross to watch the animation. In 1869‚ he extracted a substance from white blood cells that he called nuclein. What do you think he was actually extracting? 2. Frederick Griffith http://biology.clc.uc.edu/courses/Bio104/dna.htm Frederick Griffith’s famous experiment was conducted in 1928. In his experiment‚
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DNA synthesis inhibitors Nucleic acid inhibitors are chemicals which inhibit the production of nucleic acids including both DNA and RNA. DNA and RNA inhibitors inhibit enzyme action in DNA replication in the same way as the topoisomerase inhibitors we discussed earlier. Topoisomerase inhibitors (1) (10) are chemicals which interfere with the enzymes that allow DNA strands to separate and to re-join‚ a process that is required for the division of bacteria and without which DNA cannot effectively
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process by which DNA turns into polypeptides is a complicated and long. Two main steps in changing the DNA into a polypeptide are transcription and translation‚ with transcription coming first. The process first starts in the nucleus of the cell. The DNA begins to unfold with the help of a helicase. During the transcription phase of the change‚ strands of DNA begin to unwind and the complementary mRNA is made or transcribed. The way they do this is by using the common pairs of DNA triplet bases (A-T
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Cell Lab Report Experiments : 1. PCR . 2. Protein extraction and purification . 3. Protein concentration determination . 4. SDS-PAGE . 1. The aim of experiments : 2.1 The aim of PCR experiment is to replicate some DNA dimmers by using specific enzymes used for replication in vitro which is done in lab not by living organisms. 2.2 The aim of protein extraction and purification experiment is to extract some proteins and purify them by specific methods.
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