Organic Lab Report Extraction Separation of a Carboxylic Acid‚ a Phenol‚ and a Neutral Substance Purpose The objective of the experiment is to identify the three substances in the mixture and to determine the percent recovery of each from the mixture. The unknown will consist of either benzoic acid or 2-chlorobenzoic acid‚ 4-t-butyl phenol. Determine the weight of crystals and also the percent yield Introduction There are various techniques of extractions‚ solid/liquid
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the Extraction lab is to separate and purify benzoic acid‚ 2-naphthol‚ and naphthalene. These organic solids are purified by partitioning the solid in 2 immiscible solvents‚ diethyl ether and sodium bicarbonate or sodium hydroxide. II. Significance a. The significance of the Extraction lab is to purify Acetanilide benzoic acid‚ 2-naphthol‚ and naphthalene by determining the partition coefficient. This value is determined by dividing the solubility of the given solute in the extraction solvent
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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DNA FINGERPRINTING LAB REPORT DNA contains genetic material and information that makes up each individual trait. Every person can be identified by providing his or her genetic information based on a particular DNA strand. DNA information is an effective way of identifying persons if it is used properly. It is used to identify humans in different situations such as crime scenes‚ accident scenes‚ paternity testing‚ soldier remain identification‚ inheritance claims‚ missing person investigations‚
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A REPORT ON THE OVER-EXTRACTION OF GROUNDWATER IN QUEENSLAND Prepared for: THE IDEAS SUMMIT ON THE ENVIRONMENT Due: 22 May 2013 Submitted: 22 May 2013 Prepared By: Katie Gray Executive Summary The purpose of this report is to focus on an aspect of environmental economics‚ namely over-extraction of groundwater in Queensland and provide and evaluate a possible solution for this issue. Groundwater is a valuable resource which is not being protected in Queensland. Its preservation
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for the DNA ligation‚ in which the gene products with sticky ends will be inserted to a plasmid vector. Also‚ to transform E.coli DH5α cells by introducing the plasmids DNA which contains the gene of interests into the E.coli strain(DH5α). The plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance to kanamycin‚ this allows for the selection of successful transformed cells. 2. Overview of experiments DNA purification
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this lab was to isolate DNA from a food sample‚ amplify the DNA using a polymerase chain reaction‚ and test the amplified DNA for the presence of the Bt gene or the 35s promoter. In part one of DNA isolation‚ the food sample was crushed before Lysis Buffer was added‚ in part to break down some cell walls‚ but also to increase area of the food sample being touched by the Lysis Buffer. The purpose of Lysis Buffer is to break down the cells in the food sample and release their DNA into the solution. By
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suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your PCR? If the Taq DNA polymerase concentration is too low‚ not all DNA fragments will be fully replicated‚ which in turn reveals very faint bands. The optimal
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TRANSCRIPTION: Transcription is the process of which DNA matches corresponding RNA bases‚ Transcription is located in the Nucleus‚ and the only type of RNA that is involved in Transcription is mRNA‚ and the purpose is so that the code can get out of the Nucleus‚ mRNA is also made through Transcription‚ It also takes information that doesn’t directly make proteins but it helps makes codes for the production of proteins‚ DNA Transcription consist of 4 nucleotide bases‚ Adenine‚ Thymine‚ Cytosine‚ Guanine
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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