Djina Jan-18-15 Lab #6 - DNA Extraction lab Introduction: DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Cytosine with Guanine. S ince DNA is the blueprint for life‚ every living thing contains DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect
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Biotechnology Lab Report Lab: Extracting DNA from Bananas and Strawberries Purpose: To properly and successfully extract DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed
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DNA EXTRACTION FROM KIWI ISGR SCIENCE Aalah Yousif‚ Ella Sobek April 27‚ 2012 Background All living organisms (plants‚ animals‚ and bacteria) are made up of cells. Any cell consists of many parts but the parts that play the key roles are the nucleus‚ cell membrane‚ and cytoplasm. The nucleus is like the “brain” of the cell (that also contains DNA). The cell membrane is like a wall that guards the cell. And the cytoplasm is a jelly-like substance which contains other smaller organelles like
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EXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes‚ DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate‚ namely: Dische Test‚ Murexide Test‚ Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleic
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What is gel electrophoresis? Gel electrophoresis is a technique that separates pieces of DNA (or other biological molecules) by size. Pieces of DNA in a test tube all look the same. DNA Gel Electrophoresis Gel electrophoresis separates pieces of DNA by size so that researchers can further analyze them BURST Training Session November 29‚ 2005 Once the DNA samples are loaded onto the gel‚ an electric current is applied to the gel. DNA is negatively charged due to all the phosphate groups in the
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Gel electrophoresis is a routine laboratory procedure in biochemical studies that takes advantage of a protein’s amphoteric nature to determine its molecular weight and charge by running the sample through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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Brandon Schmetterer 3-13-15 Biology labs DNA Extraction Lab DNA is extracted from humans for genetic testing‚ for body identification‚ and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next‚ the cells must be burst open in order to release DNA. Third‚ DNA is separated from protein and debris. Lastly‚ the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis
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Extraction of DNA from Cheek Cells Gene Smith February 29th 2013 INTRODUCTION DNA‚ deoxyribonucleic acid‚ is the genetic material of every living organism and is found in the nucleus of eukaryotic cells. DNA is often called the ‘blueprint for life’ because it contains the necessary information to carry out all the living processes of the cell (1).The purpose of this lab was to extract DNA from human cheek cells. The isolated DNA could be used inmapping or sequencing‚ PCR‚ crime scene investigation
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