Discussion Although only the extraction of strawberry DNA was performed in this lab‚ this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well‚ and compare it to the steps taken in the strawberry protocol. As described in the procedure using strawberries‚ the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective
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land objects and water objects. Numerical algorithms have been identified and devised to segment and classify remote sensing imagery and LiDAR data into land and water pixels‚ to form and enhance land and water objects‚ and to trace and vectorize the boundaries between land and water objects as shoreline features. A contouring routine is developed as an alternative method for extracting shoreline features from LiDAR data. While most of numerical algorithms are implemented using C++ programming language
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DNA DNA‚ or Deoxyribonucleic Acid‚ is described‚ in Encarta Encyclopedia as a genetic material of all cellular organisms and most viruses. DNA carries the information needed to direct protein synthesis and replication. Protein synthesis is the production of the proteins needed by the cell or virus for its activities and development. Replication is the process by which DNA copies itself for each descendant cell or virus‚ passing on the information needed for protein synthesis. In most cellular
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Extraction of Caffeine from Tea Leaves Jaybee Balilea‚ Sharmaine Baysic‚ and Maria Anjelette Patricia Belen 3BIO7‚ Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Extraction is a technique for isolating and purifying organic substances. In this technique‚ a solution is combined thoroughly with a second solvent that is immiscible with the first solvent. The solute is extracted from one solvent into the other because it is more soluble in the second solvent
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Extraction of Caffeine from Tea Leaves using Dichloromethane (CH2Cl2) Abstract The extraction of caffeine (C8H10N402) was done in two parts. The first procedure used was solid-liquid extraction which was done by boiling the tea leaves in 100 ml. distilled water for 5-10 minutes. The filtrate was acquired and was extracted again by liquid-liquid extraction using dichloromethane (CH2Cl2). The combined DCM (CH2Cl2) was washed and dried to yield crude caffeine. The crude caffeine was purified
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Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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Extraction of metals The History of Metals. Metals have been used by people for many thousands of years. Gold and silver‚ found as native metal‚ were used both as jewellery and as a status symbol - nothing new there. These metals were known in the Stone Age‚ but gold and silver are too soft to be used as tools. The first really useful metal to be discovered was bronze. This began the Bronze Age. Bronze was used extensively for tools and weapons in Asia and Africa from 4‚500 B.C. (6‚500 years
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DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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Extraction Report For experience 4c we were supposed to determine which one is the organic layer in three different tubes that has two layers. To determine which is aqueous (water) or organic‚ I used the technique of adding droplets of water to each tube. If the layer is water‚ then the drops of added water will dissolve in the aqueous layer and increase it’s volume. If the added water form droplets or a new layer‚ then it is the organic layer. Tube 1 (water and n-butyl chloride)‚ after adding
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with its Laundry detergent bar‚ RIN. The overall objective of the marketing planning was to differentiate and position RIN‚ a non-soap detergent bar‚ as a fabric washer that gives consumers a superior cleaning power with much less effort than what is currently obtainable in the market. Lever ‘s seeks to gain an entry into the laundry soap segment where it currently does not have product offering. The Company hopes to capitalize on the success in its two brands of Non-soap Detergent powders‚ Surf
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