DNA Barcoding Click on the following link and view the background on DNA barcoding: https://www.dnalc.org/resources/animations/dna-barcoding.html 1. What is a DNA barcode? DNA barcoding is a fast accurate method of identifying plants and animals‚ or products made from them. DNA barcode is DNA sequence that uniquely identifies each species of living things. Short DNA sequences are used to identify species by comparing them with the known barcodes in large databases. When you get to the section where
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SOP: Solid phase extraction of cylindrospermopsin in filtered water samples Document identifier: SOP_TOXIC_UDU_05F Prepared by: James S. Metcalf and Geoffrey A. Codd‚ UDU Date: 7 July 2005 1 Introduction In order to determine the concentration of cylindrospermopsin in the extracellular fraction of filtered environmental waters‚ solid phase extraction (SPE) is necessary to concentrate the toxin to concentrations capable of being detected by HPLC. 2 Experimental 2.1 Materials
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Extraction and Sublimation of Caffeine from Tea Leaves By: Ashley Barnes CH 236 – P8 Experiment 4 & 4.5 Due: 10/15/2014 Table 1. Table of Reagents Discussion The extraction yield refers to the percent of caffeine in the tea leaves at start of the extraction experiment. Tea leaves had 0.55 grams of mass per eight ounce serving. The weight of the extracted crude caffeine was 0.264 grams. Therefore‚ the extraction yield was 48%. This means that we pulled out almost half of the caffeine
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is green‚ when an alcohol is oxidized by the reagent. A change in color of the reagent from orange to green represents a positive test. Primary alcohols are oxidized by the reagent to carboxylic aicds; secondary alcohols are oxidized to ketones. Tertiary alcohols are not oxidized at all by the reagent. Hence‚ this reaction can be used to distinguish tertiary alcohols form primary and secondary alcohols. Procedures: 1. Place ethanol‚ isopropyl alcohol and tert-butyl alcohol in three different
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Alcohol Facts and statistics ( www.niaaa.nih.gov-2012 ) Global Burden of alcohol abuse‚ In 2012‚ 3.3 million deaths‚ or 5.9 percent of all global deaths (7.6 percent for men and 4 percent for women)‚ were attributable to alcohol consumption.. Alcohol contributes to over 200 diseases and injury-related health conditions‚ most notably alcohol dependence‚ liver cirrhosis‚ cancers‚ and injuries. In 2012‚ alcohol accounted for 5.1 percent of disability adjusted life years (DALYs) worldwide. Globally‚
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Strawberry DNA lab Extraction Purpose: The purpose of this lab experiment is to see if this particular type of fruit which in this case is a strawberry contains DNA. Using materials such as a Ziploc bag to help keep the entire strawberry in one place while it gets smashed and test tube to help better see the DNA and break down of the liquid in the left over strawberry sediments. Background information: The long thick fibers of DNA store the information for the function of the chemistry
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“Team Marketplace Assignment” “Tide vs. Store Brand” Laundry detergent is a very necessary item and can be found in many varieties at local grocery stores. However‚ some brands tend to overpower others. During our team marketplace assignment we compared two different detergents at two different locations. We compared Tide and the generic brand detergent at both Harris Teeter and Food Lion. We compared the differences and similarities of each brand. We also observed the positioning‚ prices‚ promotions
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p-dichlorobenzene from its mixture using acid-alkaline extraction. 2. To determine the percentage recovery and melting point of benzoic acid and p-diclorobenzene. Apparatus:Separatory funnel(250mL)‚Buchner funnel‚beaker. Materials:Benzoic acid‚p-dichlorobenzene‚ether‚10% NaOH‚conc.HCl‚distilled water‚ anhydrous CaCl2. Introduction: Organic compounds in an aqueous mixture can be separated by shaking the aqueous solution with a solvent which is immiscible with water. Extraction involves dissolving
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main source of evidence. Notoriously unreliable‚ these techniques have since faded away to the stunning reliability of DNA forensics. In 1984‚ British geneticist Alec Jeffreys of the University of Leicester discovered an interesting new marker in the human genome. Most DNA information is the same in every human‚ but the junk code between genes is unique to every person. Junk DNA used for investigative purposes can be found in blood‚ saliva‚ perspiration‚ sexual fluid‚ skin tissue‚ bone marrow‚ dental
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DNA sequencing From Wikipedia‚ the free encyclopedia [pic] The term DNA sequencing refers to sequencing methods for determining the order of the nucleotide bases—adenine‚ guanine‚ cytosine‚ and thymine—in a molecule of DNA. Knowledge of DNA sequences has become indispensable for basic biological research‚ other research branches utilizing DNA sequencing‚ and in numerous applied fields such as diagnostic‚ biotechnology‚ forensic biology and biologicalsystematics. The advent of DNA sequencing
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