EXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes‚ DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate‚ namely: Dische Test‚ Murexide Test‚ Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleic
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DNA EXTRACTION FROM KIWI ISGR SCIENCE Aalah Yousif‚ Ella Sobek April 27‚ 2012 Background All living organisms (plants‚ animals‚ and bacteria) are made up of cells. Any cell consists of many parts but the parts that play the key roles are the nucleus‚ cell membrane‚ and cytoplasm. The nucleus is like the “brain” of the cell (that also contains DNA). The cell membrane is like a wall that guards the cell. And the cytoplasm is a jelly-like substance which contains other smaller organelles like
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Brandon Schmetterer 3-13-15 Biology labs DNA Extraction Lab DNA is extracted from humans for genetic testing‚ for body identification‚ and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next‚ the cells must be burst open in order to release DNA. Third‚ DNA is separated from protein and debris. Lastly‚ the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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The Amplification DNA Extraction from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method
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extract DNA from a strawberry using limited household items. This was accomplished with the prior knowledge that the DNA is stored in the nucleus of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants
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Extraction of DNA from Cheek Cells Gene Smith February 29th 2013 INTRODUCTION DNA‚ deoxyribonucleic acid‚ is the genetic material of every living organism and is found in the nucleus of eukaryotic cells. DNA is often called the ‘blueprint for life’ because it contains the necessary information to carry out all the living processes of the cell (1).The purpose of this lab was to extract DNA from human cheek cells. The isolated DNA could be used inmapping or sequencing‚ PCR‚ crime scene investigation
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Detergents A detergent is a surfactant or a mixture of surfactants with "cleaning properties in dilute solutions."[1] These substances are usually alkylbenzenesulfonates‚ a family of compounds that are similar to soap but are more soluble in hard water‚ because the polar sulfonate (of detergents) is less likely than the polar carboxyl (of soap) to bind to calcium and other ions found in hard water. In most household contexts‚ the term detergent by itself refers specifically to laundry detergent
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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Cell Lab Report Experiments : 1. PCR . 2. Protein extraction and purification . 3. Protein concentration determination . 4. SDS-PAGE . 1. The aim of experiments : 2.1 The aim of PCR experiment is to replicate some DNA dimmers by using specific enzymes used for replication in vitro which is done in lab not by living organisms. 2.2 The aim of protein extraction and purification experiment is to extract some proteins and purify them by specific methods.
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