Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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DCITATION: http://www.epa.gov/dfe/pubs/laundry/techfact/keychar.htm Surfactants * Positive Environmental Characteristic: Biodegrade readily to compounds with low toxicity. Example: Straight carbon chain compounds like linear alcohol ethoxylates or betaine esters. * Key Characteristics of Concern: Toxicity to aquatic organisms‚ like fish (vertebrates)‚ daphnids (invertebrates) and algae; persistence in the environment; toxicity of biodegradation byproducts. * Example: Alkylphenol ethoxylates--biodegrade
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appliance. - For optimum performance and safety‚ please read these instructions carefully. - Before connecting‚ operating or adjusting this appliance‚ please read the instructions completely. Please keep this manual for future reference. Read Before Using SAFETY PRECAUTIONS Washing Instructions Optional Functions Maintenance Troubleshooting Installation Instructions 02 04 14 22 23 27 31 NA-148VB3_ENG.indb 1 2011-3-14 18:07:19 Consider the Environment Disposing of packaging The packaging
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We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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DNA Extraction Lab 1/17/14 Purpose : This lab was conducted in order to show and analyze the way DNA is extracted. Hypothesis: If the lab is conducted properly then we should be able to view a visible amount of DNA from the strawberry and detergent mixture. Variables: The independent variable in this experiment is the strawberry mixture while the dependent
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General process information Detergents are manufactured using a synthetic surfactant in place of the metal fatty acid salts that are used in soaps. Most of the powder detergents have soap in their mixture of ingredients‚ however it generally functions more as a foam depressant than as a surfactant. Powder detergents are manufactured using various processes‚ such as spray drying‚ agglomeration‚ dry mixing or a combination of these. Spray Drying Process The different stages / operations performed
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3.2.4 Genomic DNA extraction of the root tips After the treatment of the onion bulbs with lake water sample at different period of time‚ the DNA from the root tips will be ready to be extracted and observed. First of all‚ the genomic of the Allium cepa will be easily done by the already available Uneasy® Plant Mini kit (Qiagen‚ Germany) in the laboratory. All of the procedures can be performed by referring to the manual provided by the kit. A very small amount of root tips that weigh 100 mg will
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chance to work regarding Detergent. A detergent is an agent used for cleaning. Detergents are synthetic surfactants‚ which is produced from petrochemicals rather than fatty acids and oils. They are very effective in hard‚ soft as well as salt water. Detergents are the salts of long chain of hydrocarbons such as alkyl sulphates. A detergent has several advantages over soaps in which we can see in the following topics. Detergents are commonly said to be synthetic detergents because it is produced
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Laundry detergents are found in every household and used for removing stains and oils from clothes so they could be reused. The production of this invention first started in the United States in the 1930s but started becoming very popular only after the Second World War because the military required a chemical to remove the dirt and oil off their military outfits (Clean Living). Laundry detergents have a combination of chemicals which combat different barriers to clean the fabric. They have different
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and quantify the components‚ using paper (stationary phase – the part of the apparatus that does not move with the sample) and chromatography solvent that was 90% petroleum ether and 10% propanol (mobile phase – liquid that carries the components). A light pencil line is drawn across the strip of paper (about 2cm from one end) and the other is attached to the splint‚ making sure that the paper doesn’t touch the bottom of the beaker. The leaf is placed into boiling water for a minute and then mortared
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