what they are. It is DNA. It is one of the greatest biological discoveries in the history of mankind. It is not only related to biology but is tied to the study of chemistry as well because of the convoluted molecular structure. DNA is short for the molecule deoxyribonucleic acid. RNA or ribonucleic acid is another nucleic acid derived from DNA and used as a template to make proteins‚ the product of the genetic code. In an article‚ “What is DNA?” written by James Randerson‚ DNA is described as‚ “.
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Objective: DNA is analyzed by agarose gel electrophoresis after being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to
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ALU-HUMAN DNA TYPING THROUGH PCR Abstract This experiment is a qualitative experiment which shows if an individual has a specific dimorphic Alu element. We used a process called Polymerase Chain Reaction (PCR) to identify this Alu element. Introduction Knowing whether or not an individual possesses a certain gene can be very important in scientific research. Do to this importance PCR allows scientist to locate these Alu’s relatively easy. Our variables in this experiment were the hairs of
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BioLab3 DNA Structure and Function Lab Report Student Name: I. DNA Structure Define the following terms: Purines A colorless crystalline compound with basic properties‚ forming uric acid on oxidation. Pyrimidines A colorless crystalline compound with basic properties; a substituted derivative of this‚ especially the bases thymine and cytosine present in DNA. Nucleotides A compound consisting of a nucleoside linked to
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DIFFERENCES BETWEEN PLASMID AND CHROMOSOMAL DNA IN BACTERIA. Eukaryotes have two or more chromosomes‚ prokaryotes such as bacteria possess a single chromosome composed of double-stranded DNA in a loop. DNA is located in the nucleoid of the cell and is not associated with protein A plasmid is an extra-chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently of the chromosomal DNA. Plasmids usually occur naturally in bacteria. A chromosome
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the role of covalent bonds and hydrogen bonds in the structure of DNA. The role of covalent and hydrogen are as follows. hydrogen bonds‚ being easy to break‚ allow the DNA to break so that copies can be made. Whereas covalent bonds keep the sugar and phosphate together‚ which allows proper placement and structure. 3. Relate the role of the base pairing rules to the structure of DNA. The bases in a strand of DNA relate to the base pairing rule due to the combination of GC
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The Importance of DNA Evidence What is DNA? Is it these winding strands that look like ladders or is it what gives a person blonde hair and blue eyes? Actually‚ DNA is both of these things. DNA is a person’s genetic makeup–their hereditary blueprint passed on by their parents. It is a part of almost every cell in the human body. In each cell‚ a person’s DNA is the same; it stays the same throughout their lifetime. DNA is found in skin tissue‚ sweat‚ bone‚ the root and shaft of hair‚ earwax‚ mucus
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determining paternity to name a few. DNA based techniques are a sub-discipline of forensic biology and are a crucial component in this field of work. DNA analysis began in the mid 1980’s and revolutionised the field of forensic science. With continual refinement of DNA analysis methods in crime laboratories over the years‚ small amounts of blood‚ saliva‚ skin cells and other biological material can now be used to develop leads and confirm or disprove an account of the crime. DNA (deoxyribonucleic acid) is
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box in a class 2 promoter. He got a piece of DNA that had a good class 2 promoter and a piece of DNA that he knew he could cut with restriction endonucleases. The idea is to pair a piece of DNA with label on only one strand. In this case you want the label on the crick strand. You would take this piece of DNA with a hot phosphate on 5 prime end of the crick strand and denature it. The probe DNA helps you see where txn starts. You take that probe DNA and you mix it with transcript thats made by the
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Chapter 9 DNA-Based Information Technologies Multiple Choice Questions 1. DNA cloning: the basics Page: 307 Difficulty: 1 Ans: C Restriction enzymes: A) act at the membrane to restrict the passage of certain molecules into the cell. B) are highly specialized ribonucleases that degrade mRNA soon after its synthesis. C) are sequence-specific DNA endonucleases. D) are very specific proteases that cleave peptides at only certain sequences. E) catalyze the
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