DNA Aim This practical procedure allows you to amplify a 460 basepair fragment of DNA from within the control region of the mitochondrial genome. This can be done using three water baths or‚ if one is available‚ a thermal cycler (PCR machine). After it has been amplified‚ the DNA is run on an electrophoresis gel. Note: This method has been adapted from one developed by the Dolan DNA Learning Center at Cold Spring Harbor Laboratory. More details are available from the DNA Learning Center’s
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LABORATORY REPORT SBT 1013: INTRODUCTION TO BIOTECHNOLOGY Semester 4 Sessions 2013/2014 ID NUMBER AND NAME 1. SITI NURFATINI BINTI MOHAMAD ROSLAN (E20131007560) 2.NURHAFIZAH BINTI SAMSUDDIN (E20131007584) 3.MUNIRAH BINTI HARON (E20131007556) 4.NORSYAWANI BINTI SUPIAN (E20131007577) LECTURER : CIK FATIMAH EXPERIMENT NO. : EXPERIMENT NO.1 TITLE : EXTRACTION OF DNA DATE & DAY : 24 JUNE 2014 TITLE: EXTRACTION OF DNA OBJECTIVE: Extract DNA from
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advances in harvesting mitochondrial DNA. “Markers” are used to trace ancestry. These markers are found through DNA Sequencing and SNP testing. The general acceptance is that the human race stemmed from a woman referred to as “Mitochondrial Eve.” It is estimated that she lived 200‚00 years ago in Africa (Rice University). Margit M. K. Nass and Sylvan Nass are accredited in discovering Mitochondrial DNA‚ or mtDNA in 1960 using electron microscopy (Rice University). DNA is found in two places in the cell;
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LAB REPORT MEDICAL IMAGING (BMB 44303/ BMB 44304) SEMESTER 1’13 LABORATORY INVESTIGATE THE ULTRASOUND IMAGING NAME: MOHD AFIQ AIMAN BIN MAT ARIFFIN ID NO: 51216111078 GROUP: LO1 TITLE: Ultrasound images A) Objectives: 1. To make the students get familiar with ultrasound machine. 2. To identify image produced by ultrasound. 3. To measure the diameter/ dimension of image subject. B) Procedures: Please simplify procedures of using ultrasound machine
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Abstract: This report presents the reason why the ocean does not freeze in the winter. Since the ocean consists of salt water‚ an experiment was done to test the effect of freezing on salt water compared to pure water. The experiment was conducted three times in order to obtain accurate results and eliminate errors. In addition to the salt and pure water experiment‚ a variation of this experiment was created to test other options. In the variation experiment the effect of freezing on sugar water
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Background on Genomic DNA Isolation and Purification Generally‚ all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA (by RNAses‚ salt or other methods). Choosing which method to use will depend on many selection factors including: DNA is isolated from proteins by several methods including digestion of proteins by the enzyme proteinase K. Proteins are removed subsequently by salting-out‚ organic extraction‚ or binding of the DNA to a solid-phase support
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BioLab3 DNA Structure and Function Lab Report Student Name: I. DNA Structure Define the following terms: Purines A colorless crystalline compound with basic properties‚ forming uric acid on oxidation. Pyrimidines A colorless crystalline compound with basic properties; a substituted derivative of this‚ especially the bases thymine and cytosine present in DNA. Nucleotides A compound consisting of a nucleoside linked to
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The Gene Lab 1. Introduction The purpose of this report is to inform dog breeders of a very common genetic disease known as Progressive retinal atrophy (PRA). The report will also inform buyers of the disease so they are aware of the disorder and they can check to dog’s pedigree for the disease. PRA could affect any dog‚ as a result of this it is recommended that the offspring should be examined by a board-certified veterinary ophthalmologist and screened for the existence of the disorder.
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Mallory Charland February 9‚ 2015 Mutations of E. coli Lab Report Advanced Biology Deducing Mutations of the Lac Operon of E. coli Abstract: In this lab we determined a possible mutation in unique bacterial strains of E. coli by observing the proper responses of wild type E. coli Lac Operon as a control group. Mutated strains of E. coli were placed in four test tubes‚ each containing a different substance (lactose‚ glucose‚ water and lactose and glucose)
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genome is a circular molecule ‚ have little non-coding DNA‚ and have their genes tightly packed with few intergenic regions between genes. Furthermore‚ mitochondria are organelles that contain a separate set of DNA from the rest of the cell and the body. Mitochondrion DNA contains 16‚569 nucleotides and contains 37 genes. These genes are all involved in the production of energy and storage of ATP. The point of this lab is to understand how DNA can mutate over
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