Discussion Although only the extraction of strawberry DNA was performed in this lab‚ this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well‚ and compare it to the steps taken in the strawberry protocol. As described in the procedure using strawberries‚ the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective
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The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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Tan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube‚ spatula‚ glass rod‚ dropper‚ beaker‚ test tube rack‚ skewer. Materials : - 1 egg‚ meat tenderizer‚ salt‚ water ‚ soap‚ isopropyl alcohol 91%‚ pineapple juice. Variables : Manipulated Variable : Responding
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Gel electrophoresis is a procedure which sorts molecules based on size and charge. The gel in gel electrophoresis refers to the object that separates the molecules. Electrophoreses refers to the force that is used to move the molecules through the gel. There are 2 stages to gel electrophoresis‚ separation and visualization. During separation the gel matrix is placed in an electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel
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LABORATORY REPORT BIOLOGY CELL AND GENETICS (SB10203) TITLE: DNA Extraction from Banana DATE OF LABORATORY: 03/05/2013 LECTURER’S NAME: DR. LAM NYEE FAN DEMONSTRATORS’ NAME: MISS NOR EZANI AHMAD MISS LUSIA BAREK MOSES LABORATORY ASSISTANT NAME: MISS ROSILAH MOHD IDRUS STUDENT NAME AND MATRIC NUMBER: ELYAS ERIC HUIL(BS12110134) BONG SIN NENG(BS12110054) EDILAH NADRAH JOHANY( DIASSOFIA PAULA FRANKIE INTRODUCTION DNA is present in the
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for the DNA ligation‚ in which the gene products with sticky ends will be inserted to a plasmid vector. Also‚ to transform E.coli DH5α cells by introducing the plasmids DNA which contains the gene of interests into the E.coli strain(DH5α). The plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance to kanamycin‚ this allows for the selection of successful transformed cells. 2. Overview of experiments DNA purification
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DNA replication mechanism is an integral and the most important part of cell division. It is a complicated mechanism that requires a lot of steps and different enzymes. Many scientists were trying to understand the mechanism of DNA replication and its role in the transfer of the hereditary information to the offsprings. DNA replication begins with a single origin of replication that starts off the process by creating a replication bubble. In many eukaryotic cells‚ the DNA strand will have more than
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TRANSCRIPTION: Transcription is the process of which DNA matches corresponding RNA bases‚ Transcription is located in the Nucleus‚ and the only type of RNA that is involved in Transcription is mRNA‚ and the purpose is so that the code can get out of the Nucleus‚ mRNA is also made through Transcription‚ It also takes information that doesn’t directly make proteins but it helps makes codes for the production of proteins‚ DNA Transcription consist of 4 nucleotide bases‚ Adenine‚ Thymine‚ Cytosine‚ Guanine
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Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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The investigation on the average mass of DNA with the mass of banana‚ strawberry and kiwi. Purpose: The purpose of this lab is to investigate the comparison between the amount of DNA per gram of fruit that can be extracted from a banana‚ strawberry and kiwi and to determine which one has more DNA. Hypothesis: The banana genome contains 837 MBPs and the strawberry genome contains 206 MBPs and the kiwi genome contains 128 MBPs. This states that there are more base pairs in a banana genome
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