Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
Premium DNA Bacteria Molecular biology
Fingerprint Process Fingerprinting is an important process that many employers require in order for a person to obtain a job. Fingerprinting clearance is done to check a person’s background to make sure that he or she has never been convicted of a crime that would prevent him or her from obtaining the job in question. Jobs like teachers and day-care workers need to be fingerprinted to ensure that children who come into contact with these professionals are being taught and taken care of by someone
Free Crime Criminal law
done to examine both strains of Halobacteria in the aqueous solutions. DNA extraction was done to take DNA from the Halobacteria strains and examine their genotypes. The PCR reaction’s purpose was to locate specific
Premium Bacteria Microbiology DNA
Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
Premium Polymerase chain reaction DNA Molecular biology
Introduction During the course of this lab‚ we explored whether or not certain processed foods contained Genetically Modified Organisms. Genetically modified food is an important subject in the world today. Ever since Flavr Savr came out with their tomatoes grown from genetically modified seeds in 1994 genetically modified foods has become ever more popular amongst distributors of produce (Mestel‚ 2013). The world’s population has grown by around one billion in the last decade (US Bureau of the
Premium Polymerase chain reaction Molecular biology Genetically modified food
Strawberry DNA Extraction Donovan Roberts Mrs. Caudill Honors Biology 3/7/11 Introduction The structure of DNA is made of nitrogenous bases‚ phosphate groups‚ and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus‚ bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells can
Premium DNA
BRAIN FINGERPRINTING TECHNOLOGY Mandar Ghate Department Of Computers‚ Padmabhushan Vasantdada Patil Pratisthans College Of Engineering mandarghate4@gmail.com Abstract— Brain fingerprinting is a new computer-based technology to identify the perpetrator of a crime accurately and scientifically by measuring brain-wave responses to crime-relevant words or pictures presented on a computer screen. Brain fingerprinting has proven 100% accurate in over 120 tests‚ including tests on FBI agents‚ tests
Premium Brain Forensic evidence Electroencephalography
Presence of Alu element within PV92 on chromosome 18 in a mixed ethnical population and its connection with humans’ ancestral origins By Shiyu Song Biology 155Q LAB-A: 14:30-17:30 Dr. Jacob April 17th 2013 Abstract The Alu insertion (+) within PV92 on chromosome 16 usually reflects a person’s ancestral origin. This study aimed to find the correlation between the presences of Alu insertion (+) within PV92 on chromosome 16 within a population. We used PCR to detect the Alu insertions
Premium Genetics DNA Gene
Proteomics is the study of proteomes‚ which are groups of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate
Premium Protein DNA Gene
Agarose Gel Electophoresis of DNA Topoisomers Introduction DNA can exist as different isomers that change the confirmation of the DNA’s structure. DNA can be in a linear confirmation this is a relaxed confirmation as the DNA can rotate about its axis unconstrained. It can also exist as a nicked circle this is also a relaxed confirmation as the DNA strands can again rotate freely with respect to one another. Covalently closed circular DNA or cccDNA exists as a supercoil this is because the covalent
Premium DNA