temperature on the activity of lipase Aim: To investigate the effects of temperature on the activity of lipase enzyme on milk which contain fats or lipids. Introduction: Enzyme is a kind of biological catalyst that made up of protein. It can speed up the metabolic reactions on various kinds of substances. Like lipase can break down lipid into glycerol or fatty acids in milk. Since enzyme is made up of protein which easily affected by varies temperature. This experiment is carried out to find the
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Enzymes What Are Enzymes? Substances that speed up chemical reactions are called catalysts. Organic catalysts are called enzymes. Enzymes are specific for one particular reaction or group of related reactions. Many reactions cannot occur without the correct enzyme present. They are often named by adding "ase" to the name of the substrate. Example: Dehydrogenases are enzymes that remove hydrogen. Induced-fit Theory The shape of the enzyme must match the shape of the substrate
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Enzyme action is the simple mechanism by which enzymes catalyse chemical reactions. This begins with the binding of the substrate to the active site on the enzyme. The binding of the substrate to the enzyme causes changes in the distribution of electrons in the chemical bonds of the substrate. This then causes the reactions that lead to the formation of products that are then released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique shape that
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Introduction: Enzymes are biological catalysts that permit cells to carry out the many functions that are required in a living system. Every enzyme has a specific substrate and a specific function. Enzymes alter substrates one of three ways: by adding something to the substrate‚ removing something from the substrate‚ or by changing its conformation‚ otherwise known as its shape. The structure of an enzyme and its ability to function exists because it binds to an active site‚ thus increasing the
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Enzyme Reaction to Variable Elizabeth Alvidrez University of Texas at El Paso Abstract: An enzyme is a protein that serves as a biological catalyst (Denniston‚ 2007). Metabolic reactions happen with in cells. Enzymes are proteins that are used to speed up these reactions without being consumed by them (Mader‚ 2010). Catalase is a catalyst that digests potent hydrogen peroxide and converts it into H2O and O (Campbell Reese‚ 2008). The environment plays an important role
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Factors Affecting The rate of Enzyme Activity Prediction: As the temperature increases the rate of enzyme activity will also increase‚ thus increasing the rate of reaction. However‚ if the temperature is too high the enzyme will denature. Materials: 4 test tubes 2 small beakers A dozen filter paper disks Test tube rack Hydrogen peroxide (H2O2) Potato extract Forceps Thermometer Hot plate Large beaker Ice cubes Graduated cylinder Stopwatch Procedure: Step 1 Place 10 mL of potato
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Enzymes Ashley Njeru Mrs. Booth Tuesday‚ March 19th 2013 SBI 4U INTRODUCTION: The cellular activity of all organisms is controlled through the use of enzymes. An enzyme is a special type of biological molecule‚ usually a protein‚ which speeds up a chemical reaction; most are soluble in water or a dilute salt solution. There are about 4000 different enzymes in a typical living cell. If even one of these enzymes is missing or
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this lab was to troubleshoot causes related to PCR components and develop an experiment that would test if the Taq amount is suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your PCR? If the Taq DNA polymerase
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Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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however‚ without enzymes‚ these reactions could possibly take several years to complete. An enzyme is a macromolecule‚ generally a protein‚ that lowers the activation energy of a reaction without being changed by the reaction‚ and this causes the reaction to occur much faster than usual (Campbell et al.‚ 2014). The act of speeding of a chemical reaction is called catalysis‚ and molecules that perform this are called catalyst. Enzymes act as catalysts‚ and there a many types of enzymes that perform many
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