Discussion The primary purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity
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Affect of enzyme concentration to the rate of reaction Aim: With the experiment of protein solution‚ in this case egg white added to different pepsin concentrations (0%‚ 0.2%‚ 0.4%‚ 0.6%‚ 0.8%‚ 1.0%) shows‚ as the egg white is a protein and the pepsin works as an enzyme‚ how a higher pepsin concentration and therefore a larger amount of enzymes effect the rate of reaction. Hypothesis: An increased concentration of pepsin speeds up the time the mixture needs to come clear. Introduction:
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Enzymes are biological molecules (proteins) that act as a catalyst and help complex reactions occur everywhere in life‚ for example a piece of steak that is being digested into energy. Molecules found at the beginning of the process are called substrates‚ and these enzymes exchange them into differing molecules known as products. Nearly all-metabolic processes in a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply
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The environmental factors that effected the rate of enzyme reactions were the enzyme concentration‚ pH‚ and temperature. These environmental factors help enzymes break down the poisonous chemicals into harmless substance. When we tested the liver with 2ml of hydrogen peroxide for a normal reaction it showed that it was exothermic. We added more hydrogen peroxide and the reaction rate of the liver was 3. We learned that the catalase is reusable because the liver reacted both times when we put in
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LABORATORY REPORT BIOLOGY CELL AND GENETICS (SB10203) TITLE: DNA Extraction from Banana DATE OF LABORATORY: 03/05/2013 LECTURER’S NAME: DR. LAM NYEE FAN DEMONSTRATORS’ NAME: MISS NOR EZANI AHMAD MISS LUSIA BAREK MOSES LABORATORY ASSISTANT NAME: MISS ROSILAH MOHD IDRUS STUDENT NAME AND MATRIC NUMBER: ELYAS ERIC HUIL(BS12110134) BONG SIN NENG(BS12110054) EDILAH NADRAH JOHANY( DIASSOFIA PAULA FRANKIE INTRODUCTION DNA is present in the
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TRANSCRIPTION: Transcription is the process of which DNA matches corresponding RNA bases‚ Transcription is located in the Nucleus‚ and the only type of RNA that is involved in Transcription is mRNA‚ and the purpose is so that the code can get out of the Nucleus‚ mRNA is also made through Transcription‚ It also takes information that doesn’t directly make proteins but it helps makes codes for the production of proteins‚ DNA Transcription consist of 4 nucleotide bases‚ Adenine‚ Thymine‚ Cytosine‚ Guanine
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Temperature and Enzyme Activity Aim: To investigate the effect of temperature on enzyme activity Hypothesis: As the temperature deviates from 40°C the activity will lower Equipment: – Chemicals: – Milk – Junket tablets – Hot water – Ice – Test tubes – Stopwatch – Measuring cylinder Risk Assessment: |Hazard |Risk |Prevention | |Hot Water
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Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the
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certain environmental factors affect the enzyme activity rate. For the first experiment‚ where we tested the increase in concentration of enzyme with the substrate‚ we found that higher concentration of enzyme increases the rate of reaction of the enzyme. This is because more enzyme molecules are present‚ which allow more substrate molecules to get into the active sites of the enzyme (Sattler W& Esterbauer H). When calculating the absorbance of different enzyme concentration‚ it was noticeable that
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Enzymes are proteins that increase or decrease the rate of chemical reactions. They are generally globular proteins and are around 62 amino acids residues in size. What enzymes do is determined by their 2-dimensional shape. A lot of enzymes are bigger than the substrate they act on‚ but only a little part of the enzyme involved directly with the catalysis. Without enzymes the chemical reactions in the body‚ would be so slow‚ the body would shut down. And cell reactions would take too much energy
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