Name __________________________________ AP Biology Period _________ Date ______________________ AP: LAB-RELATED AP EXAM ESSAYS LAB 1. OSMOSIS AND DIFFUSION ESSAY 1992 A laboratory assistant prepared solutions of 0.8 M‚ 0.6 M‚ 0.4 M‚ and 0.2 M sucrose‚ but forgot to label them. After realizing the error‚ the assistant randomly labeled the flasks containing these four unknown solutions as flask A‚ flask B‚ flask C‚ and flask D. Design an experiment‚ based on the principles of diffusion
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expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate that responds with an enzyme is used to view the antibody/protein complex. Within a muscle tissue‚ there could be as many as 19 different
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Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
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PTC Testing Lab 11/12/13 Abstract: The main purpose of this lab is to determine that you have the dominant PTC gene or recessive PTC gene. PTC testing is a method used to test for a genetic trait. People who have dominant gene taste PTC (phenylthiocarbamide)‚ and people who have recessive do not taste PTC. This trait is passed genetically from parents to their children‚ so that if a person has the trait‚ then at least one of their parents had the trait as well (New York
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order to analyze a dna molecule in a molecular biology lab you must determine the length in nucleotide pairs. Electrophoresis is an extremely useful tool in order to compare the mobility on agarose gels with dna markers of known lengths. Dna is a polymer that is negatively charged due to the sugar phosphates. When dna is on an electric field such as the electrophoresis gel the different lengths of dna migrate at different rates when they move through the porous gel. The ends of the gel are marked with
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DNA profiling is a method of identifying an individual by unique characteristics of their DNA. A specific DNA pattern‚ called a profile‚ is obtained from an individual or a sample of tissue. This allows the comparison of the base sequence of two or more DNA samples to determine whether they are related. DNA profiling has many uses‚ in prevention of economic fraud‚ dietetic work‚ and classifying species‚ identifying bodies‚ forensic science‚ screening for disease‚ and investigating paternity.
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DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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Fingerprint Process Fingerprinting is an important process that many employers require in order for a person to obtain a job. Fingerprinting clearance is done to check a person’s background to make sure that he or she has never been convicted of a crime that would prevent him or her from obtaining the job in question. Jobs like teachers and day-care workers need to be fingerprinted to ensure that children who come into contact with these professionals are being taught and taken care of by someone
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of this lab was to compare the action of a catalyst (enzyme) under different environmental conditions. This was determined by performing a variety of different experiments. The first experiment was performed by adding hydrogen peroxide to sand. Due to the fact that the sand was not soluble in the hydrogen peroxide‚ no reaction thus no catalyst were present. Manganese dioxide was also added to the hydrogen peroxide creating a moderately fast reaction thus leading to believe that an enzyme was present
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done to examine both strains of Halobacteria in the aqueous solutions. DNA extraction was done to take DNA from the Halobacteria strains and examine their genotypes. The PCR reaction’s purpose was to locate specific
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