Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity cheryl yelton November 30‚ 2014 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2.
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scene by comparing with the DNA samples. Polymerase Chain Reaction(PCR) is used to amplify the small amount of Deoxyribonucleic Acid (DNA) for forensic or genetic studies‚ which require necessary product and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using an electric current applied to the gel matrix‚ which causes the DNA samples to move towards
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It was hypothesized that if the animal was larger‚ then it would have larger hemoglobin. It was thought that larger mammals would need more oxygen-rich hemoglobin to support healthy function. This lab did not support this hypothesis. The results in table 1 displayed that the hemoglobin of all mammalian samples traveled about the same distance. This trend can be seen in graph 1 as the mammalian hemoglobin samples migrated the same distance. Because the proteins traveled around the same distance it
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Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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isolated. Isolating DNA from the Pure Colonies Grown In addition‚ DNA were isolated from the colonies grown and its concentration was 33.6 ng/uL and the A260/280 value was 1.84. Next‚ an agarose gel electrophoresis was conducted to see if any DNA fragments were present. A band was visible and that validates that genomic DNA has been isolated. PCR Amplification to Determine Strain of P. larvae with ERIC-Specific Primers ERIC-specific primers were used to genotype the genomic DNA of P. larvae isolated
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Now You See It – Copper Cycle Lab The purpose of the lab is to discover what happens when someone executes a series of procedures‚ beginning with copper metal. What is done | What is observed | 1. Started with copper‚ Cu (s). | reddish‚ brownish‚ orange-ish‚ powder-like | 2. Added nitric acid‚ HNO3 (aq). | acid turns blue and smells like chlorine. | 3. Added water‚ H2O (l). | stayed the same | 4. Added sodium hydroxide‚ NaOH (aq). | changed consistency‚ gel-like | 5. Heated the mixture
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Activity 1.3.1: DNA Detectives Introduction Bones can provide a snapshot of the identity of a person- they can predict height‚ stature‚ gender‚ ethnicity and even age. However‚ it is what lies inside these hard calcified tissues‚ the DNA housed inside the body’s cells‚ that holds the key to true genetic identity. Tissue is made up of many cells‚ the building blocks of life. Tucked inside the body’s cells‚ you will find chromosomes. These structures house your genes and contain the DNA code necessary
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macromolecules that compose all life? BellworkIf your DNA is 23.5 % adenine. How much Guanine is there? Chapter 12- DNA Structure and Replication The Recipe Book Nucleic Acids- Function Nucleic acids function is storing‚ copying‚ and transmitting instructions for making proteins. DNA and RNA are nucleic acids!!! Monomer of Nucleic Acids- Nucleotides Think of DNA as a recipe book What is DNA and how does it work? DNA Storage DNA stores instructions for making proteins The total human
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Part B: Practical Report The Effect of Temperature on Enzyme Activity Aim: To investigate how temperature effects the enzyme catalase. Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water‚ when compared to cold water. Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner. Introduction: An enzyme is a protein‚ which speeds up a specific chemical reaction without altering
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