Restriction Enzymes and Electrophoresis Bhumik Patel Phillips 1/16/11 Restriction enzymes are tools in DNA research that can cut DNA into exactly needed pieces. Certain cuts can be rough‚ while others can be clean. Certain cuts can have an organized pattern to have a staggered cut. Other cuts will leave complementary bases with them. Electrophoresis allows the manipulation of DNA to separate and organize those parts. Electrophoresis is the substrate electric movement of the separation
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Objective: DNA is analyzed by agarose gel electrophoresis after being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to
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AGAROSE GEL ELECTROPHORESIS Gel electrophoresis is a widely used technique for the analysis of nucleic acids and proteins. Most every molecular biology research laboratory routinely uses agarose gel electrophoresis for the preparation and analysis of DNA. We will be using agarose gel electrophoresis to determine the presence and size of PCR products. Background: Electrophoresis is a method of separating substances based on the rate of movement while under the influence of an electric field
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2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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anthropologists ran a gel electrophoresis with DNA from Skeleton 3 and two missing persons‚ Julia Ly and Teresa Chen to help in DNA identification. This process would allow restriction enzymes to cut by a specific restriction site and run through the gel‚ where the DNA fragments would move from the negative side to the positive side of the gel due to the negative charge of the phosphate group in DNA. The smaller the DNA fragments‚ the further they move down the gel. As mentioned above‚ the DNA that was collected
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DNA as a Key Witness Criminals‚ often unknowingly‚ leave parts of themselves behind. These pieces are not always visible to the untrained eye. Hair‚ skin‚ blood‚ and fingerprints all contain elements that are unique to each person. It is with DNA testing and fingerprinting‚ that criminals can be identified and crimes can be linked. This system of testing and matching has become the “most essential and reliable method of catching criminals” in the United States (Lynch 67). Advancing technology
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DNA fingerprinting is a way of identifying a specific individual‚ rather than simply identifying a species or some particular trait. It is also known as genetic fingerprinting or DNA profiling. As a technology‚ it has been around since at least 1985‚ when it was announced by its inventor‚ Sir Alec Jeffreys. DNA fingerprinting is currently used both for identifying paternity or maternity and for identifying criminals or victims. There is discussion of using DNA fingerprinting as a sort of personal
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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to that person‚ therefore it is a great resource for police to help locate people involved in a case. Families share many of the same traits in their DNA but people are unsure of whether or not they have similarities‚ In this experiment that question will be answered. There are three main fingerprint types: arches‚loops‚and whorls. (GeneEd - DNA Fingerprints 2003‚ April 12). The police take fingerprint samples from the crime scene or from involved people). Once the fingerprints are taken and
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Ligation of Lambda DNA pre-digested with EcoRI and HinDIII. Restriction of Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme used‚ determined
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