University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Enzyme Catalysis Lab: Laboratory Write Up Problem/Question: What happens to the amounts of gas produced in a catalyzed reaction of Hydrogen Peroxide when the catalyst is mixed with an acid? Theory/Hypothesis: In this experiment the researcher and their team will be performing tests on a catalyst (proteins found in potato) to see what will happen when the catalyst in a Hydrogen Peroxide reaction is treated with an acid before the reaction occurs. The researcher hypothesizes that the after treating
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May 1‚ 2013 Enzymes as Drug Targets Enzymes are defined as any of numerous proteins produced in living cells that accelerate or catalyze the metabolic processes of an organism. Enzymes are usually very selective in the molecules that they act upon‚ called substrates‚ often reacting with only a single substrate. The substrate binds to the enzyme at a location called the active site just before the reaction catalyzed by the enzyme takes place. Enzymes can speed up chemical reactions by up to
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LAB REPORT GUIDELINES Writing a good lab report is an important goal of your science education‚ and gives you the opportunity to enhance your writing skills and to communicate your understanding of the scientific process to others. Your lab report for this semester will be a write up of your independent research project. This will follow the standard format for a lab report and should include the following sections: Title Introduction Materials and Methods Results Discussion Conclusion
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[Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on the desired
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BACK TITRATION- DETERMINATION OF THE CARBONATE CONTENT IN GARDEN LIME NAME: OSEI BONSU ERIC ID: 3906409 EXPERIMENT: I.2.2.1.
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The Scientific Method in Relation to Fingerprinting Although no one scientist is recognized as the inventor of the scientific method‚ its use in the scientific community believes to date back to 384-322 B.C. During this time‚ Aristotle recognized the importance of deriving reliable knowledge based on observation. As time progressed to the 1200s‚ Roger Bacon acknowledged the significance of the repeating cycle of observation‚ hypothesis‚ experimentation and verification‚ a scientific method that
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reproductive. Orientation is when the organism is placed in their beneficial environment consisting of two behaviors‚ taxis (movement directly towards or away something) and kinesis (random movement). Reproductive is detected using drosophila in this lab‚ it consists of finding courting and mating with another of the species Agnostic behavior is found in a situation where the animal feels threated by another‚ leading to the organism looking bigger or more threatening to the opponent. Within the Agnostic
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is the process where non sex cells reproduce by duplicating DNA and dividing into two identical cells. Each cell has a complete set of chromosomes. These cells are called diploid (double) as they have twice as many chromosomes as the two cells that join via fetilization to form it. Meiosis is the process where sex cells (called gametes which carry the genetic material for deteriming the sex of an offspring) reproduce by duplicating DNA and diving into four new cells that have half (haploid) the
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