Introduction: Through a series of labs‚ we were able to extract and isolate our mitochondrial DNA‚ nuclear DNA‚ and cellular DNA to then amplify a part of an intron in the tissue plasminogen activator gene (TPA) through Polymerase Chain Reaction (PCR) and run on an agarose gel to detect the presence or absence of a transposable element known as an Alu element. The main purpose of this experiment was to use our class as a population‚ to test whether or not we were in Hardy-Weinberg Equilibrium. The
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Introduction! ! The Michelson Interferometer is commonly used to determine the wavelength of light or measure very small distances. It was invented by Albert Abraham Michelson and is commonly used in optical interferometry‚ a branch of physics involving a family of techniques one could use to extract information about waves by superimposing them. ! ! The original application of the Michelson Interferometer was to the famous Michelson-Morley experiment in 1887. Prior to Einstein’s
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The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
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reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP) is a metal-containing enzyme that serves as a good model for the study of metal ion interactions in enzyme catalysis. Native E. coli ALP contains three
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baking Yeast helps raise the dough and also yeast is used in the process of making wine.The Reason why yeast was chosen to was to see how yeast can metabolize different sugars and how much Co2 they release when when metabolizing.To test the yeast at the lab we used 4 different types of carbohydrates which are known as Glucose‚Lactose‚Sucrose‚and Starch.The main thing that make some of these carbohydrates different from each other is if it’s a Monosaccharide‚ Disaccharide‚or Polysaccharide. Monosaccharide
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Introduction The purpose of this lab was to calculate and determine the optimal dosage of ultraviolet (UV) light to expose Saccharomyces cerevisiae (S. cerevisiae) to that would result in a survival rate of 20-40%. Saccharomyces cerevisiae commonly referred to as baker’s yeast is a single-celled eukaryote. Yeast is capable of growing as either a haploid or a diploid cell. The diploid cell can reproduce mitotically or sporulate‚ a process initiating meiosis and forming an ascus with four spores (Sacchromyces
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2014/15 Module name and number Molecular Biology & Genetic Engineering: BS941 Assignment title “DNA sequencing: where we are and where it’s going” Student Number 1464986 Word Count 2310 The article focuses on the advances achieved in DNA sequencing by first providing a brief background on DNA‚ and how it was initially sequenced. The paper then takes into consideration four of the major DNA sequencing techniques. These include: Sanger’s Chain Termination Method‚ Pyrosequencing‚ Single Molecule
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EXPERIMENT NO. 1 INTRODUCTION TO LAB INSTRUMENTS. 1. BREADBOARD We should be familiar to the following things about a breadboard: * What is a breadboard and what is it used for? * How does it work? * Setting Up. * Limitations. What is a breadboard and what is it used for? A breadboard (or protoboard) is usually a construction base for prototyping of electronics. The term "breadboard" is commonly used to refer to a solderless breadboard (plugboard). It was designed by
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TITLE OF EXPERIMENT 2 : DILUTION 2.1: OBJECTIVES Upon completion of this experiment‚ students should be able to: 1. determine the concentration of coloured solution such as FeCl3 by using dilution and colour differentiating (colorimetric) techniques. 2.2: INTRODUCTION Concentration can be expressed in many different ways such as percentage volume and percentage weight etc. In laboratory‚ normally concentrations were expressed as molarity and normality. Molarity is the
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Abstract: Bacteria consists of a large domain of prokaryotic microorganism in which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly
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